PMID: 6984342Dec 22, 1982Paper

Human lymphocyte membrane proteins treated with neuraminidase

Biochimica Et Biophysica Acta
H GmünderW Lesslauer

Abstract

Human peripheral blood lymphocytes were surface-iodinated, treated with neuraminidase from Vibrio cholerae and lysed with non-ionic detergent. In addition, surface membrane fractions were isolated from surface-iodinated cells in the absence of detergents and treated with neuraminidase after membrane isolation. The effect of neuraminidase treatment on the membrane proteins was studied by two-dimensional gel electrophoresis. One surface-labelled protein of 45 000 molecular weight which is characterized by its association with the detergent-resistant matrix of the cells and by its specific enrichment in an isolated membrane fraction, was found to be particularly sensitive to neuraminidase treatment both of intact cells and isolated membranes. A prominent labelled protein of apparent molecular weight of 60 000 is observed in the soluble fraction after neuraminidase treatment of intact cells. The analogous protein is detected when isolated membrane fractions are treated with neuraminidase.

References

Mar 9, 1976·Biochemistry·H Furthmayr, V T Marchesi
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L C AnderssonH Wigzell
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M J OwenM J Crumpton
Nov 1, 1977·The Journal of Experimental Medicine·P P Jones
May 25, 1978·Nature·J Flanagan, G L Koch
Jun 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·A Novogrodsky, E Katchalski

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Citations

Jul 2, 1984·European Journal of Biochemistry·H Gmünder, W Lesslauer

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