Human lymphocyte proliferation. I. Correlation between activated and proliferating T-lymphocytes

Immunology Letters
F KristensenA L de Weck

Abstract

The response of human peripheral blood lymphocytes to Con A and PHA has been analyzed by [3H]thymidine incorporation and cytofluorometry. Using the latter method, it is possible to quantitate the number of cells in the G0 phase (normal RNA and DNA content) and in the G1 phase (elevated RNA, but normal DNA content). A very high correlation is found between numbers of Con A or PHA-induced G1 cells and [3H]thymidine incorporation in healthy donors. This high correlation is found when culture medium is enriched with 10% autologous plasma or 10% AB-serum. The use of a recently developed defined serum-free medium (RPMI 1640 with albumin, alanine, transferrin, sodium selenite and zinc chloride), however, suggest that donors can be divided into two groups according to different medium requirements for PHA-stimulated lymphocytes. Because several immunoregulatory mechanisms at the level of T-lymphocytes take place in the G1 phase, it can therefore be expected that cytofluorometric analyses of lymphocytes in the various cell cycle phases may improve the interpretation of altered lymphocyte response to lectins and antigens.

References

Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·Z DarzynkiewiczM R Melamed
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·Z DarzynkiewiczM R Melamed
May 1, 1982·Immunobiology·F KristensenA L de Weck

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Citations

Jan 1, 1991·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·J E BaierD Ricken
Jan 1, 1992·Pharmacology & Therapeutics·U Bjare
Jan 1, 1984·International Journal of Immunopharmacology·Y WangA L de Weck
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·W ShiveK S Matthews
Jul 1, 2009·Immunology and Allergy Clinics of North America·Priska LochmatterWerner J Pichler
Feb 19, 2002·Oncogene·Arturo P SaavedraJudy L Meinkoth

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