Human mast cells (HMC-1 5C6) enhance interleukin-6 production by quiescent and lipopolysaccharide-stimulated human coronary artery endothelial cells.

Mediators of Inflammation
Damandeep S WaliaK N Dileepan

Abstract

We examined the effect of intact human mast cells (HMC-1 5C6) and their selected mediators on interleukin-6 (IL-6) production and bone morphogenetic protein-2 (BMP-2) expression in human coronary artery endothelial cells (HCAEC) in the presence and absence of lipopolysaccharide (LPS). Scanning electron microscopy showed that HMC-1 5C6 cells adhere to HCAEC in cocultures. Addition of HMC-1 5C6 cells markedly enhanced the IL-6 production by quiescent and LPS-activated HCAEC even at the maximal concentration of LPS. Furthermore, mast cell-derived histamine and proteases accounted for the direct and synergistic effect of mast cells on IL-6 production that was completely blocked by the combination of histamine receptor-1 antagonist and protease inhibitors. Another novel finding is that histamine was able to induce BMP-2 expression in HCAEC. Collectively, our results suggest that endotoxin and mast cell products synergistically amplify vascular inflammation and that histamine participates in the early events of vascular calcification.

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Citations

Nov 20, 2012·Journal of Cell Communication and Signaling·Tarak SrivastavaChristopher J Harrison
Apr 5, 2016·Immunity, Inflammation and Disease·Ida WaernSara Wernersson
Jan 22, 2013·Pharmacology & Therapeutics·Simon KennedyPasquale Maffia
Jun 5, 2016·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Hong Ju ShinYoung Hwan Park
Nov 14, 2013·PloS One·Anne Marijn van der GraafMarijke M Faas
Jan 24, 2018·Cardiovascular Research·Mélanie LambertFabrice Antigny
Sep 8, 2017·Frontiers in Immunology·Jason M SpringerKottarappat N Dileepan

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Methods Mentioned

BETA
PCR
scanning electron microscopy

Software Mentioned

Primer Express

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