Human matrix attachment regions insulate transgene expression from chromosomal position effects in Drosophila melanogaster.

Molecular and Cellular Biology
S J NamciuR E Fournier

Abstract

Germ line transformation of white- Drosophila embryos with P-element vectors containing white expression cassettes results in flies with different eye color phenotypes due to position effects at the sites of transgene insertion. These position effects can be cured by specific DNA elements, such as the Drosophila scs and scs' elements, that have insulator activity in vivo. We have used this system to determine whether human matrix attachment regions (MARs) can function as insulator elements in vivo. Two different human MARs, from the apolipoprotein B and alpha1-antitrypsin loci, insulated white transgene expression from position effects in Drosophila melanogaster. Both elements reduced variability in transgene expression without enhancing levels of white gene expression. In contrast, expression of white transgenes containing human DNA segments without matrix-binding activity was highly variable in Drosophila transformants. These data indicate that human MARs can function as insulator elements in vivo.

References

Apr 1, 1992·Current Opinion in Genetics & Development·U K LaemmliY Adachi
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R A McKnightL Hennighausen
Sep 15, 1988·Biochemical and Biophysical Research Communications·M H HofkerR R Frants
Mar 5, 1988·Journal of Molecular Biology·E IzaurraldeU K Laemmli
Jan 1, 1995·Progress in Nucleic Acid Research and Molecular Biology·B Levy-Wilson
Oct 25, 1994·Nucleic Acids Research·L PoljakU K Laemmli
Aug 1, 1996·Chromosoma·P L StrisselH Swift
Feb 1, 1997·Plant Molecular Biology·A H van der Geest, T C Hall
Feb 1, 1996·Methods : a Companion to Methods in Enzymology·E S Dieken, R E K Fournier
Dec 13, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·P Rollini, R E Fournier
Jul 1, 1990·Plant Physiology·M A ConklingH M Goodman

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Citations

May 14, 1999·Current Opinion in Genetics & Development·A C Bell, G Felsenfeld
May 3, 2013·PloS One·Olga KyrchanovaPavel Georgiev
Nov 9, 2001·Annual Review of Genetics·T I Gerasimova, V G Corces
Jan 18, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Alex H Chang, Michel Sadelain
Apr 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Parimal Majumder, Haini N Cai
Apr 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Rebeca Carballar-LejarazúAnthony A James
Oct 23, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Alasdair MackenzieJon Martin Collinson
Jul 6, 2007·Applied Microbiology and Biotechnology·Tianyun WangYafeng Wang
Jun 29, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Félix Recillas-TargaCatherine M Farrell
Feb 13, 2001·FEBS Letters·J Yamamura, K Nomura
Aug 23, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Satoshi WatanabeKoji Akasaka
Jan 17, 2007·Transgenic Research·Fabrice GuillemotMarie-Dominique Devignes
Nov 26, 2010·The Journal of Clinical Investigation·Patrick G GallagherDavid M Bodine
Dec 21, 2010·Plant Cell Reports·Stacy D SingerZongrang Liu
Jul 30, 2015·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·William A MacDonaldMellissa R W Mann
Aug 20, 2003·The Journal of Cell Biology·Keith Byrd, Victor G Corces
Feb 6, 2004·DNA Sequence : the Journal of DNA Sequencing and Mapping·Susan M Wykes, Stephen A Krawetz
Dec 3, 2003·Molecular and Cellular Biology·Michaël WeberThierry Forné
Jul 28, 2016·G3 : Genes - Genomes - Genetics·Alisha V JohnPatricia J Wittkopp
Oct 28, 2017·Journal of Cellular and Molecular Medicine·Zheng-Wei TianGuang-Hua Xu
Mar 25, 2005·Bioorganicheskaia khimiia·A V SassE D Sverdlov

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