Human medulloblastoma gangliosides

Glycobiology
F ChangS Ladisch

Abstract

To establish a model system for the study of ganglioside metabolism of the human brain tumor, medulloblastoma, we have chemically characterized the gangliosides of the Daoy cell line. These cells contain a high concentration of gangliosides (143 +/- 13 nmol LBSA/10(8) cells). The major species have been structurally confirmed to be GM2 (65.9%), GM3 (13.0%), and GD1a (10.3%). Isolation of individual gangliosides homogeneous in both carbohydrate and ceramide moieties by reversed-phase HPLC and analysis by negative-ion fast atom bombardment collisionally activated dissociation tandem mass spectrometry have allowed us to unequivocally characterize ceramide structures. In the case of GM2, 10 major ceramide subspecies were identified: d18:1-hC16:0, d18:1-C16:0, d18:0-C16:0, d18:1-C18:0, d18:1-C20:0, d18:1-C22:0, d18:2-C24:1, d18:1-C23:1, d18:1-C24:1, and d18:1-C24:0. Taken together with previous studies, these findings in buman medulloblastoma cells support the view that high expression and marked heterogeneity of ceramide structure are general characteristics of tumor gangliosides, molecules which are shed by the tumor cells and which are biologically active in vivo.

Citations

Apr 16, 2002·Journal of the American Society for Mass Spectrometry·Peter B O'ConnorCatherine E Costello
May 17, 2003·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Pam FredmanThomas Brezicka
Oct 17, 2009·Expert Review of Neurotherapeutics·Joseph R MoskalGlyn Dawson
Apr 9, 2005·International Journal of Cancer. Journal International Du Cancer·Mepur H RavindranathDonald L Morton
Dec 6, 2007·Journal of Neurochemistry·Roger A KroesJoseph R Moskal
May 20, 2006·Journal of Neuro-oncology·P WielgatK Zwierz
Jul 31, 2021·Current Treatment Options in Oncology·Zahraa F AudiSanaa M Nabha

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