Abstract
In this study we have investigated, using three different human melanoma cell lines (M1Do., M3Da., M4Be.). the varying capacity of melanoma cells to induce platelet aggregation in the presence or absence of inhibitors of ADP or thrombin. The expression levels of different integrins (alpha v, beta 3, alpha v beta 3, alpha IIb, alpha v beta 3) were evaluated by immunoprecipitation, binding and flow cytometry studies. The level of ADP in supernatants of melanoma cells were quantified by ADP bioassay and HPLC. Platelets were irreversibly aggregated by M3Da, as shown by electron microscopy, in contrast to M1Do, which induced a slow reversible aggregation. M4Be. did not induce platelet aggregation. In both cases, with M3Da. or M1Do., apyrase but not PPACK inhibited platelet induced aggregation. An anti-alpha v beta 3 monoclonal antibody (LYP18) or polyclonal antibody inhibited platelet aggregation. A similar number of LYP18 molecules bound to the surface of M1Do., M3Da. and M4Be. cell lines. Biological HPLC assays of ADP present in the supernatant of tumour cell lines showed the highest concentration of ADP to be secreted by M3Da., followed by M1Do., and none detected for M4Be. These results show that differences in in vitro aggregat...Continue Reading
References
Jul 1, 1992·Experimental Cell Research·K V HonnK K Nelson
Apr 3, 1992·Cell·R O Hynes
Mar 28, 1986·Science·R PytelaE Ruoslahti
May 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·I M GrossiK V Honn
Oct 1, 1989·American Journal of Clinical Pathology·B C McGregorR A Warnke
May 16, 1988·Biochemical and Biophysical Research Communications·A Di PietroD C Gautheron
Nov 15, 1988·Biochimica Et Biophysica Acta·G L Nicolson
Sep 1, 1988·Journal of Cellular Physiology·K A KnudsenG P Tuszynski
Nov 16, 1988·Journal of the National Cancer Institute·K E UgenK J Kao
Jan 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L WeissK V Honn
Apr 1, 1988·The Journal of Clinical Investigation·S KarpatkinB S Coller
Jan 15, 1988·European Journal of Biochemistry·H Boukerche, J L McGregor
Jun 1, 1987·Thrombosis Research·J D ScarlettC J Louis
Jul 25, 1986·Science·M J HumphriesK M Yamada
Feb 1, 1972·British Journal of Haematology·J F MustardM A Packham
May 1, 1973·International Journal of Cancer. Journal International Du Cancer·G J GasicS Murphy
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·E PearlsteinS Karpatkin
Apr 1, 1980·The Biochemical Journal·D W Mason, A F Williams
Jun 1, 1982·Analytical Biochemistry·S H Payne, B N Ames
Citations
Jan 1, 1996·Journal of Cancer Research and Clinical Oncology·E HeinmöllerH Zirngibl
Aug 1, 1995·Thrombosis Research·L OleksowiczE Puszkin
May 28, 2014·Thrombosis Research·David StegnerBernhard Nieswandt
Nov 8, 2015·Life Sciences·Chien-Kei WeiChin-Chung Wu
Aug 8, 2007·Thrombosis Research·María José Santos-MartínezMarek W Radomski
Oct 20, 2004·British Journal of Pharmacology·Paul JuraszMarek W Radomski
Feb 9, 2008·Journal of Cellular Biochemistry·Ozge UluçkanKatherine Weilbaecher
Nov 3, 2010·Journal of Thrombosis and Haemostasis : JTH·N M Bambace, C E Holmes
Jan 1, 2014·Journal of Cellular Physiology·Deva SharmaChris E Holmes
Sep 26, 2013·Blood·Elisabeth M BattinelliJoseph E Italiano
Jun 18, 2011·Blood·Elisabeth M BattinelliJoseph E Italiano
Oct 14, 2014·Frontiers in Oncology·Laura AmoSusana Larrucea
May 8, 2016·Blood·Raphael Leblanc, Olivier Peyruchaud
Aug 21, 2013·Critical Reviews in Oncology/hematology·Lutfi SuleimanHabib Boukerche
Jul 25, 2017·Cancers·Omar ElaskalaniPat Metharom
Sep 29, 2017·Cancers·Marion LavergnePierre H Mangin
Feb 20, 2013·Oncology Letters·Lian LianMin Tao
Jul 7, 2017·Blood·Min Soon ChoVahid Afshar-Kharghan
Jul 25, 2019·Cells·Vitor H AlmeidaRobson Q Monteiro
Oct 31, 2017·Convergent Science Physical Oncology·Joanna L SylmanOwen J T McCarty
Aug 20, 2016·Oncotarget·Rongfeng ZhangYang Liu
Jan 21, 2017·Frontiers in Cell and Developmental Biology·Claire K S MeikleRandall G Worth
Jul 7, 2017·Cancer Metastasis Reviews·Michael LamDavid G Menter
Jul 15, 2017·Cancer Metastasis Reviews·P GreseleM Sebastiano
Nov 18, 2018·Cancers·Léa PlantureuxLaurence Panicot-Dubois
Oct 13, 2020·Frontiers in Oncology·Serena Lucotti, Ruth J Muschel
Feb 15, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Satoshi TakagiIrene M Ghobrial
Feb 9, 2021·ACS Omega·Zhujing HaoYun-Long Xia
Jul 10, 2020·Translational Research : the Journal of Laboratory and Clinical Medicine·Ann S KimKeith R McCrae
May 4, 2021·Blood·Derrick L TaoOwen J T McCarty
Oct 1, 2021·Frontiers in Oncology·Ana Luisa Palacios-AcedoLaurence Panicot-Dubois