PMID: 7527241Aug 1, 1994Paper

Human melanoma cell lines differ in their capacity to release ADP and aggregate platelets

British Journal of Haematology
H BoukercheJ L McGregor

Abstract

In this study we have investigated, using three different human melanoma cell lines (M1Do., M3Da., M4Be.). the varying capacity of melanoma cells to induce platelet aggregation in the presence or absence of inhibitors of ADP or thrombin. The expression levels of different integrins (alpha v, beta 3, alpha v beta 3, alpha IIb, alpha v beta 3) were evaluated by immunoprecipitation, binding and flow cytometry studies. The level of ADP in supernatants of melanoma cells were quantified by ADP bioassay and HPLC. Platelets were irreversibly aggregated by M3Da, as shown by electron microscopy, in contrast to M1Do, which induced a slow reversible aggregation. M4Be. did not induce platelet aggregation. In both cases, with M3Da. or M1Do., apyrase but not PPACK inhibited platelet induced aggregation. An anti-alpha v beta 3 monoclonal antibody (LYP18) or polyclonal antibody inhibited platelet aggregation. A similar number of LYP18 molecules bound to the surface of M1Do., M3Da. and M4Be. cell lines. Biological HPLC assays of ADP present in the supernatant of tumour cell lines showed the highest concentration of ADP to be secreted by M3Da., followed by M1Do., and none detected for M4Be. These results show that differences in in vitro aggregat...Continue Reading

References

Jul 1, 1992·Experimental Cell Research·K V HonnK K Nelson
May 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·I M GrossiK V Honn
Sep 1, 1988·Journal of Cellular Physiology·K A KnudsenG P Tuszynski
Jan 1, 1988·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L WeissK V Honn
Apr 1, 1988·The Journal of Clinical Investigation·S KarpatkinB S Coller
Feb 1, 1972·British Journal of Haematology·J F MustardM A Packham
May 1, 1973·International Journal of Cancer. Journal International Du Cancer·G J GasicS Murphy
Apr 1, 1980·The Biochemical Journal·D W Mason, A F Williams

❮ Previous
Next ❯

Citations

Jan 1, 1996·Journal of Cancer Research and Clinical Oncology·E HeinmöllerH Zirngibl
May 28, 2014·Thrombosis Research·David StegnerBernhard Nieswandt
Aug 8, 2007·Thrombosis Research·María José Santos-MartínezMarek W Radomski
Oct 20, 2004·British Journal of Pharmacology·Paul JuraszMarek W Radomski
Feb 9, 2008·Journal of Cellular Biochemistry·Ozge UluçkanKatherine Weilbaecher
Nov 3, 2010·Journal of Thrombosis and Haemostasis : JTH·N M Bambace, C E Holmes
May 8, 2016·Blood·Raphael Leblanc, Olivier Peyruchaud
Jul 25, 2017·Cancers·Omar ElaskalaniPat Metharom
Sep 29, 2017·Cancers·Marion LavergnePierre H Mangin
Jul 7, 2017·Blood·Min Soon ChoVahid Afshar-Kharghan
Oct 31, 2017·Convergent Science Physical Oncology·Joanna L SylmanOwen J T McCarty
Jan 21, 2017·Frontiers in Cell and Developmental Biology·Claire K S MeikleRandall G Worth
Jul 7, 2017·Cancer Metastasis Reviews·Michael LamDavid G Menter
Jul 15, 2017·Cancer Metastasis Reviews·P GreseleM Sebastiano
Oct 13, 2020·Frontiers in Oncology·Serena Lucotti, Ruth J Muschel
Feb 15, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Satoshi TakagiIrene M Ghobrial
Jul 10, 2020·Translational Research : the Journal of Laboratory and Clinical Medicine·Ann S KimKeith R McCrae
May 4, 2021·Blood·Derrick L TaoOwen J T McCarty
Oct 1, 2021·Frontiers in Oncology·Ana Luisa Palacios-AcedoLaurence Panicot-Dubois

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.