Human Memory CD4+ T Cell Immune Responses against Giardia lamblia

Clinical and Vaccine Immunology : CVI
Christina Skår SaghaugKurt Hanevik

Abstract

The intestinal protozoan parasite Giardia lamblia may cause severe prolonged diarrheal disease or pass unnoticed as an asymptomatic infection. T cells seem to play an important role in the immune response to Giardia infection, and memory responses may last years. Recently, TH17 responses have been found in three animal studies of Giardia infection. The aim of this study was to characterize the human CD4(+) T cell responses to Giardia. Peripheral blood mononuclear cells (PBMCs) were obtained from 21 returning travelers with recent or ongoing giardiasis and 12 low-risk healthy controls and stimulated in vitro with Giardia lamblia proteins. Production of tumor necrosis factor alpha (TNF-α), gamma interferon, interleukin-17A (IL-17A), IL-10, and IL-4 was measured in CD4(+) effector memory (EM) T cells after 24 h by flow cytometry. After 6 days of culture, activation and proliferation were measured by flow cytometry, while an array of inflammatory cytokine levels in supernatants were measured with multiplex assays. We found the number of IL-17A-producing CD4(+) EM T cells, as well as that of cells simultaneously producing both IL-17A and TNF-α, to be significantly elevated in the Giardia-exposed individuals after 24 h of antigen sti...Continue Reading

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Nov 20, 2015·Clinical and Vaccine Immunology : CVI·Steven M Singer
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Related Concepts

IL17A protein, human
Teens
Antigens, Protozoan
Flow Cytometry
Giardiasis
Immunologic Memory
Interferon Type II
Nongranular Leukocyte
Lymphocyte Activation
Tumor Necrosis Factor-alpha

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