Human menstrual blood-derived stem cells promote functional recovery in a rat spinal cord hemisection model

Cell Death & Disease
Qinfeng WuChuanming Dong

Abstract

Spinal cord injury (SCI) is associated with a dismal prognosis including severe voluntary motor and sensory deficits in the presence of the current therapies, thus new and efficient treatment strategies are desperately required. Along with several advantages, such as easy accessibility, high-yield, potential of enormous proliferation, menstrual blood-derived mesenchymal stem cells (MenSCs) have been proposed as a promising strategy in regeneration medicine. In this study, the MenSCs were transplanted into incomplete thoracic (T10) spinal cord injury (SCI) rats, all rats were sacrificed at 7, 14, and 28 days after surgery. Based on the results, we found that MenSCs transplantation improved the hind limb motor function. Besides, H&E staining showed that MenSCs treatment markedly reduced cavity formation in the lesion site. Furthermore, treatment by MenSCs showed more MAP2-positive mature neurons, as well as axonal regeneration manifested by NF-200 and less expression of chondroitin sulfate proteoglycans (CSPGs) than the non-treatment in the lesion site. Additionally, immunofluorescence, Western blot, and qRT-PCR methods showed that levels of brain-derived neurotrophic factor (BDNF) were significantly higher in the injured spinal ...Continue Reading

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Citations

Jun 4, 2019·Frontiers in Cellular Neuroscience·Bernat SoriaVivian Capilla-González
Oct 14, 2020·Journal of Molecular Neuroscience : MN·Princy ChoudharySangeeta Singh
Jul 3, 2021·Metabolic Brain Disease·Xiaorong ZhaoBo Niu

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Methods Mentioned

BETA
biopsy
FACS
enzyme-linked immunosorbent assay
density gradient centrifugation
protein assay

Software Mentioned

Image
Pro Plus
SPSS Statistics
Image Pro Plus
Primer Express

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