Human mesenchymal and murine stromal cells support human lympho-myeloid progenitor expansion but not maintenance of multipotent haematopoietic stem and progenitor cells

Cell Cycle
Stefan RadtkeBernd Giebel

Abstract

A major goal in haematopoietic stem cell (HSC) research is to define conditions for the expansion of HSCs or multipotent progenitor cells (MPPs). Since human HSCs/MPPs cannot be isolated, NOD/SCID repopulating cell (SRC) assays emerged as the standard for the quantification of very primitive haematopoietic cell. However, in addition to HSCs/MPPs, lympho-myeloid primed progenitors (LMPPs) were recently found to contain SRC activities, challenging this assay as clear HSC/MPP readout. Because our revised model of human haematopoiesis predicts that HSCs/MPPs can be identified as CD133(+)CD34(+) cells containing erythroid potentials, we investigated the potential of human mesenchymal and conventional murine stromal cells to support expansion of HSCs/MPPs. Even though all stromal cells supported expansion of CD133(+)CD34(+) progenitors with long-term myeloid and long-term lymphoid potentials, erythroid potentials were exclusively found within erythro-myeloid CD133(low)CD34(+) cell fractions. Thus, our data demonstrate that against the prevailing assumption co-cultures on human mesenchymal and murine stromal cells neither promote expansion nor maintenance of HSCs and MPPs.

References

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Mar 15, 2003·Blood·Christian P KalbererAleksandra Wodnar-Filipowicz
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May 17, 2013·Stem Cells and Development·Heike WegmeyerMarkus Neubauer

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Citations

Apr 21, 2016·Cell Cycle·Nicole MendeClaudia Waskow
Aug 17, 2020·Stem Cell Research & Therapy·Ali Akbari, Jafar Rezaie

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Methods Mentioned

BETA
density gradient centrifugation

Software Mentioned

CXP
GraphPad Prism

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