Human mesenchymal stem cells as delivery of osteoprotegerin gene: homing and therapeutic effect for osteosarcoma

Drug Design, Development and Therapy
Bo QiaoDianming Jiang

Abstract

Biological treatments have been studied extensively and previous studies have proved that osteoprotegerin (OPG) can inhibit the development and progress of human osteosarcoma. However, the utility of biologic agents for cancer therapy has a short half-life, which can hardly deliver to and function in tumor sites efficiently. Mesenchymal stem cells (MSCs) have the potential to migrate to tumor sites. In this study, MSCs transfected with adenoviruses carrying the OPG gene (MSCs-OPG) were used via the tail vein to treat athymic nude mice (nu/nu) bearing osteosarcoma. In vivo and ex vivo images were used to validate the MSCs homing to tumors. The therapeutic effect for osteosarcoma was evaluated by observations on growth of tumors and bone destruction. The results showed that infected MSCs-OPG labeled with red fluorescent protein (RFP) can migrate to tumor sites and express OPG protein. The treatment by MSCs-OPG reduced the tumor growth and inhibited bone destruction in vivo. All these indicated that MSCs can deliver OPG to tumor sites, which could be a new direction of biological treatment for human osteosarcoma.

Citations

Jun 9, 2016·Expert Opinion on Biological Therapy·Anna HagenhoffPeter J Nelson
Dec 3, 2016·Future Oncology·Maliha KhanAmanda L Olson
Oct 21, 2016·Expert Opinion on Investigational Drugs·Marie-Françoise HeymannDominique Heymann
Mar 30, 2016·World Journal of Stem Cells·Ann De Becker, Ivan Van Riet
Oct 4, 2019·Clinical & Experimental Metastasis·Maria V DeligiorgiDimitrios T Trafalis
Sep 18, 2020·Tissue Engineering. Part B, Reviews·Eun-Jin LeeStella Alimperti
Jul 15, 2021·Journal of Bone Oncology·Xingyu ChangJingjing Yang

❮ Previous
Next ❯

Methods Mentioned

BETA
surgical resection
fluorescence imaging
xenografts
flow cytometry
transfection
enzyme-linked immunosorbent assay
light microscopy
ELISA

Software Mentioned

SPSS

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.