Human mesenchymal stem cells promote cancer motility and cytokine secretion in vitro

Cells, Tissues, Organs
Agmal ScherzedNorbert Kleinsasser

Abstract

Interactions of human mesenchymal stem cells (hMSC) with tumors are controversially discussed since there is evidence for both tumor progression as well as tumor inhibition by hMSC. The objective of the present study is to investigate whether hMSC support cell motility and cytokine secretion in a head and neck squamous cell carcinoma cell line (HLaC 78). A spheroid model was generated in which the ultrastructure of spheroids was analyzed using scanning electron microscopy (SEM). The migration capability was monitored in a monolayer as well as in a spheroid model. The variation in migration and secretion of interleukin (IL)-6, IL-8 and vascular endothelial growth factor (VEGF), as well as the expression of the multidrug resistance gene (MDR-1) was investigated. Finally, the alteration in the cell cycle was analyzed by flow cytometry. SEM showed a tight cell-cell contact with extensive secretion of extracellular matrix. The migration and invasion capability of HLaC 78 was enhanced by hMSC. Cancer cell motility was also increased by hMSC as well as secretion of the cytokines IL-6, IL-8 and VEGF. hMSC did not induce the expression of MDR-1 in HLaC 78, and there was no alteration in the cell cycle of HLaC 78 after cocultivation with...Continue Reading

Citations

May 23, 2015·International Journal of Oncology·Agmal ScherzadStephan Hackenberg
Dec 22, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Erhan SahinEzgi Bektur
Oct 15, 2014·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Luca AmpolliniFederico Quaini
Dec 15, 2016·Oncotarget·Beomseok SonBuHyun Youn
Aug 24, 2017·Biotechnology Journal·Luís P FerreiraJoão F Mano
Apr 7, 2021·Stem Cell Reviews and Reports·Greet MerckxEsther Wolfs
Mar 20, 2018·ACS Biomaterials Science & Engineering·Lisa A SawickiApril M Kloxin

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