Human mesenchymal stromal cells could deliver erythropoietin and migrate to the basal layer of hair shaft when subcutaneously implanted in a murine model

Tissue & Cell
P L MokO Ainoon

Abstract

Mesenchymal stromal cells (MSC) are an attractive cell-targeting vehicle for gene delivery. MIDGE (an acronym for Minimalistic, Immunologically Defined Gene Expression) construct is relatively safer than the viral or plasmid expression system as the detrimental eukaryotic and prokaryotic gene and sequences have been eliminated. The objective of this study was to test the ability of the human MSC (hMSC) to deliver the erythropoietin (EPO) gene in a nude mice model following nucleofection using a MIDGE construct. hMSC nucleofected with MIDGE encoding the EPO gene was injected subcutaneously in Matrigel at the dorsal flank of nude mice. Subcutaneous implantation of nucleofected hMSC resulted in increased hemoglobin level with presence of human EPO in the peripheral blood of the injected nude mice in the first two weeks post-implantation compared with the control groups. The basal layer of the hair shaft in the dermal layer was found to be significantly positive for immunohistochemical staining of a human EPO antibody. However, only a few basal layers of the hair shaft were found to be positively stained for CD105. In conclusion, hMSC harboring MIDGE-EPO could deliver and transiently express the EPO gene in the nude mice model. The...Continue Reading

References

May 9, 1991·The New England Journal of Medicine·A J Erslev
Oct 4, 1990·The New England Journal of Medicine·S Baskin, N Lasker
Apr 1, 1995·The Journal of Clinical Investigation·Y HamamoriL Kedes
Nov 22, 1997·Stroke; a Journal of Cerebral Circulation·T F ChoudhriD J Pinsky
May 30, 2001·Trends in Molecular Medicine·A I Caplan, S P Bruder
Aug 28, 2001·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·K U Eckardt
Mar 4, 2003·Nature Biotechnology·Steve ElliottJoan Egrie
Mar 7, 2003·Gene Therapy·H Herweijer, J A Wolff
Apr 30, 2004·The Journal of Thoracic and Cardiovascular Surgery·K H GrinnemoK Le Blanc
Jul 10, 2004·Cell Death and Differentiation·P Ghezzi, M Brines
Oct 2, 2004·The New England Journal of Medicine·Charles L BennettNicole Casadevall
May 17, 2005·Nature Materials·Hyun Joon KongDavid J Mooney
Jul 5, 2005·Journal of the American College of Cardiology·Peter van der MeerRegien G Schoemaker
Mar 9, 2006·Stem Cells and Development·Martin J HoogduijnPaul G Genever
May 5, 2006·Journal of the American Society of Nephrology : JASN·Nicoletta EliopoulosJacques Galipeau
Jan 24, 2007·Transplant International : Official Journal of the European Society for Organ Transplantation·Dingguo ZhangKejiang Cao
Apr 10, 2008·Cardiovascular Research·Ian B CoplandJacques Galipeau
Apr 16, 2008·British Journal of Haematology·Wolfgang Jelkmann
May 15, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Elise EsneaultMyriam Bernaudin
Nov 26, 2008·Molecular Immunology·Lilach LifshitzDrorit Neumann
Dec 18, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Philippe M CampeauJacques Galipeau
Mar 7, 2009·Cell Stem Cell·Jeffrey M Karp, Grace Sock Leng Teo
Jan 1, 2007·Organogenesis·K StennK Washenik
Sep 19, 2009·Cellular and Molecular Life Sciences : CMLS·Arianne Monge NaldiDaniel Bodmer
Feb 20, 2010·Circulation Research·John V TerrovitisEduardo Marbán
Mar 26, 2010·Trends in Molecular Medicine·James Ankrum, Jeffrey M Karp
Apr 27, 2010·Annual Review of Biomedical Engineering·Biju Parekkadan, Jack M Milwid
Jun 18, 2010·Journal of Dermatological Science·Bo Mi KangYoung Kwan Sung
Jul 3, 2010·Haematologica·Lilach LifshitzDrorit Neumann
Jul 16, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jason B BellPerry B Hackett
Oct 20, 2010·Journal of Dermatological Science·Bo-Young YooJung-Keug Park
Nov 16, 2010·Annual Review of Pathology·Nora G Singer, Arnold I Caplan

❮ Previous
Next ❯

Citations

Jul 4, 2015·Stem Cells and Development·Adam NowakowskiBarbara Lukomska
Jul 8, 2017·Journal of Cellular Biochemistry·Xin-Peng HanChang-Gui Wu
Apr 13, 2019·International Journal of Molecular Sciences·Shirley Suet Lee DingPooi Ling Mok
Jun 19, 2015·Biomarker Insights·Jonas Schwan, Stuart G Campbell

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.