Human Metapneumovirus Infection Inhibits Cathelicidin Antimicrobial Peptide Expression in Human Macrophages

Frontiers in Immunology
Youxian LiIngvild B Johnsen

Abstract

Human cathelicidin antimicriobial peptide (CAMP) is a critical component of host innate immunity with both antimicrobial and immunomodulatory functions. Several pathogens have been shown to downregulate CAMP expression, yet it is unclear if such modulation occurs during a viral infection. In this study, we showed that infection with human metapneumovirus (hMPV), one of the leading causes of respiratory tract infections in young children, strongly suppressed basal and vitamin-D induced CAMP expression in human macrophages. hMPV-mediated suppression of CAMP did not correlate with reduced transcriptional expression of key vitamin D signaling components, such as CYP27B1 or vitamin D receptor, suggesting a vitamin D-independent mechanism. Blocking interferon-signaling pathways did not reverse hMVP-mediated suppression of CAMP, indicating that the suppressive effect is largely interferon-independent. Instead, we identified C/EBPα as the key modulator of hMPV-mediated suppression of CAMP. hMPV infection strongly repressed the expression of C/EBPα, and a knockdown study confirmed that C/EBPα is critical for CAMP expression in human macrophages. Such modulation of CAMP (and C/EBPα) could be reproduced by TLR1/2 ligand treatment in human...Continue Reading

References

Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·V OssipowU Schibler
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·R BalsJ M Wilson
Oct 5, 2001·Clinical Microbiology Reviews·C E Samuel
Sep 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Monisha G ScottRobert E W Hancock
Jun 15, 2005·Cellular Microbiology·Peter BergmanBirgitta Agerberth
Jun 30, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Adrian F GombartH Phillip Koeffler
Aug 26, 2006·Biochemical and Biophysical Research Communications·Eva GottfriedMarina Kreutz
Jan 27, 2007·Journal of Neuroscience Research·Aroa Ejarque-OrtizJosep Saura
May 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Adrian R MartineauRobert J Wilkinson
Aug 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philip T LiuRobert L Modlin
Nov 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sif HansdottirGary W Hunninghake
Sep 15, 2009·Cell Host & Microbe·Jae-Min YukEun-Kyeong Jo
Jun 23, 2011·Peptides·Reinaldo RamosMiguel Gama
Dec 19, 2012·Cellular Immunology·Dieter VandammeLiliane Schoofs
Nov 16, 2013·Nature Chemical Biology·Ashley L HilchieRobert E W Hancock
May 6, 2014·Frontiers in Physiology·Rene F ChunMartin Hewison
May 16, 2014·Pharmaceuticals·Guangshun Wang
May 21, 2014·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Swagatika PandaSubrat Kumar
Aug 1, 2014·Journal of Cellular Physiology·Puneet DhawanSylvia Christakos
Apr 7, 2015·Trends in Immunology·Maziar DivangahiErwan Pernet
Feb 14, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Silke M CurrieDonald J Davidson
Apr 5, 2016·Immunology·Michael Bscheider, Eugene C Butcher
Apr 19, 2016·Nature Reviews. Immunology·Robert E W HancockErin E Gill
May 29, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julia SteigerMario Fabri

❮ Previous
Next ❯

Citations

May 18, 2019·Scandinavian Journal of Medicine & Science in Sports·Jonathan M ScottHeath G Gasier
May 20, 2020·Viruses·Marlies Ballegeer, Xavier Saelens

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Assay
fluorescence assay
transfection

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.