Human metapneumovirus M2-2 protein inhibits viral transcription and replication

Microbes and Infection
Yoshinori KitagawaBin Gotoh

Abstract

M2-2 protein of human metapneumovirus (HMPV) is encoded by one of two overlapping open reading frames within M2 mRNA. The precise function of HMPV M2-2 protein remains unknown. We here examined effect of M2-2 protein on HMPV transcription and replication using a minigenome construct and monitoring luciferase reporter gene expression. The minigenome assays demonstrated that M2-2 protein inhibited both transcription and RNA replication. The inhibitory function of M2-2 protein was completely abrogated by removal of eight or four amino acids from its N- or C-terminus, respectively, demonstrating importance of both short terminal sequences for maintaining its functional structure. Immunoprecipitation experiments revealed interaction of M2-2 protein with L protein, which might be involved in inhibition of HMPV transcription and replication. Prior accumulation of intracellular M2-2 protein severely restrained HMPV from replicating. Thus inherent viral control of the M2-2 gene expression in infected cells seems to be essential for efficient HMPV replication.

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Citations

Jul 4, 2012·Molecular Immunology·Tsuyoshi SugiyamaHiroshi Mori
Feb 11, 2015·The Journal of General Virology·J RenX Bao
Feb 17, 2015·Virology·Sarah L Noton, Rachel Fearns
Oct 23, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Min ZhouBin Gotoh
Jul 15, 2016·Clinical Microbiology Reviews·Pablo F CéspedesSusan M Bueno

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