PMID: 75241Feb 1, 1978Paper

Human migration inhibitory factor: purification and immunochemical characterization

The Journal of Experimental Medicine
L H BlockG Ruhenstroth-Bauer

Abstract

Using gel filtration and preparative isotachophoresis, the migration inhibitory factor (MIF) was highly purified from human lymphocytes activated with concanavalin A. MIF is an acidic protein with a mol wt of approximately equal to 25,000 daltons as determined by gel filtration and analytical polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The protein inhibits migration of macrophages in the capillary test and in addition, has a slowing effect on the electrophoretic mobility of guinea pig peritoneal macrophages. Rabbit antibodies specific for this protein, as determined by immunochemical techniques, neutralized the biological effect of MIF on migration and on the electrophoretic mobility of macrophages.

References

Sep 1, 1975·Cellular Immunology·C L GeczyA L de Weck
Sep 7, 1967·Biochemical and Biophysical Research Communications·A L ShapiroJ V Maizel
Jul 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·J R David
Feb 1, 1965·Analytical Biochemistry·C B LAURELL

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Citations

Oct 16, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·H J Schuurman, C L de Ligny
Jan 1, 1980·Journal of Immunological Methods·D E CoyleH B Halsall
Mar 1, 1996·Nature Structural Biology·M SuzukiM Sakai
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·H G RemoldA D Mednis
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·W Y WeiserJ R David
Apr 1, 1991·The American Review of Respiratory Disease·R M HoffmanR L Hardesty
Jul 15, 1980·Klinische Wochenschrift·L H BlockJ Drews
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A J WieczorekL H Block
Oct 1, 1981·Scandinavian Journal of Immunology·R A FoxK Rajaraman
Nov 15, 1982·Klinische Wochenschrift·L H BlockW Siegenthaler
Jan 1, 1982·Pharmacology & Therapeutics·J M HansonJ Morley
Mar 1, 1986·Immunology Letters·M MüllerM Kotzsch

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