Nov 1, 1992

Human milk mucin inhibits rotavirus replication and prevents experimental gastroenteritis

The Journal of Clinical Investigation
R H YolkenDavid S Newburg

Abstract

Acute gastrointestinal infections due to rotaviruses and other enteric pathogens are major causes of morbidity and mortality in infants and young children throughout the world. Breast-feeding can reduce the rate of serious gastroenteritis in infants; however, the degrees of protection offered against rotavirus infection vary in different populations. The mechanisms associated with milk-mediated protection against viral gastroenteritis have not been fully elucidated. We have isolated a macromolecular component of human milk that inhibits the replication of rotaviruses in tissue culture and prevents the development of gastroenteritis in an animal model system. Purification of the component indicates that the antiviral activity is associated with an acidic fraction (pI = 4.0-4.6), which is free of detectable immunoglobulins. Furthermore, high levels of antiviral activity are associated with an affinity-purified complex of human milk mucin. Deglycosylation of the mucin complex results in the loss of antiviral activity. Further purification indicated that rotavirus specifically binds to the milk mucin complex as well as to the 46-kD glycoprotein component of the complex. Binding to the 46-kD component was substantially reduced after...Continue Reading

  • References41
  • Citations127

References

  • References41
  • Citations127

Citations

Mentioned in this Paper

Virus Replication
Gastrointestinal Diseases
Milk, Human
Rotavirus Infections
Gastroenteritis
Neonatal Calf Diarrhea Virus
Mucins

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