Human milk oligosaccharides affect P-selectin binding capacities: in vitro investigation

Nutrition
Gabriele SchumacherChristopher Beermann

Abstract

In the initial phase of cellular immune response, selectins mediate the emigration of leukocytes from the blood stream into inflammatory regions. Human milk oligosaccharides (HMOs) possess binding epitopes of selectin ligands such as sialyl Lewis(x) and sialyl Lewis(a) and therefore might impair the interaction of selectins with cellular ligands. Neutral, acidic, sialylated, or fucosylated HMO fractions with polymerization degrees of 3 to 50 were investigated regarding this interaction in a dynamic flow chamber model that considers physiologic shear stress conditions. Human milk oligosaccharides were compared with kappa-carrageenans and pectin oligosaccharides to deduce structure-activity relations. Fucoidan and sialyl Lewis(x) served as positive controls. All HMO fractions affected P-selectin ligand binding capacity but were not comparable to fucoidan. The activity of the acidic HMO fraction resembled sialyl Lewis(x) in decreasing the binding of the ligand to P-selectin. Human milk oligosaccharides modulate rather than block the function of P-selectin.

References

Nov 15, 1989·Biochemical and Biophysical Research Communications·M P SkinnerM C Berndt
Aug 2, 1996·The Journal of Biological Chemistry·P P WilkinsR D Cummings
Jan 1, 1996·Annals of Nutrition & Metabolism·A SchwertmannC Kunz
Jul 24, 1998·Biochimica Et Biophysica Acta·J VogelU Rothe
Jan 29, 1999·Physiological Reviews·D Vestweber, J E Blanks
Feb 16, 2000·The British Journal of Nutrition·C KunzD Braun
Aug 15, 2000·Annual Review of Nutrition·C KunzS Strobel
Nov 7, 2000·Advances in Experimental Medicine and Biology·N KleinS Strobel
Oct 11, 2001·Journal of the American Chemical Society·G ThomaJ T Patton
Jun 27, 2002·European Journal of Nutrition·Silvia RudloffClemens Kunz
Oct 16, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Jean-François DeuxDidier Letourneur
Jun 28, 2003·Trends in Molecular Medicine·Klaus Ley
Oct 14, 2003·Nutrition Reviews·Bo Lönnerdal, Eric L Lien

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Citations

Dec 2, 2011·Annual Review of Food Science and Technology·Maciej ChichlowskiDavid A Mills
Sep 29, 2009·Early Human Development·Maka Mshvildadze, Josef Neu
Apr 11, 2009·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Ravisha SrinivasjoisSanjay Patole
Aug 25, 2012·BMB Reports·Kyunghun JeongJaehan Kim
Jun 11, 2014·Beneficial Microbes·S MusilovaV Bunesova
Jul 23, 2016·Carbohydrate Research·Anna Kulinich, Li Liu
Jan 17, 2016·Advances in Nutrition·YingYing HeDavid S Newburg
Oct 31, 2019·British Journal of Pharmacology·Yang CaiSaskia Braber
Apr 23, 2020·Nutrients·Magdalena Orczyk-Pawiłowicz, Jolanta Lis-Kuberka
Jun 21, 2020·Nutrients·Mostafa AsadpoorSaskia Braber
Jun 21, 2013·The American Journal of Clinical Nutrition·Gilda GeorgiBernd Stahl
Mar 16, 2016·The Biochemical Journal·Alexander J NollRichard D Cummings
Sep 27, 2018·Frontiers in Pediatrics·Veronica Ayechu-MuruzabalBelinda Van't Land
Jul 18, 2018·Frontiers in Pediatrics·Vassilis TriantisR J Joost van Neerven
Aug 22, 2008·The Journal of Nutrition·Günther Boehm, Guido Moro
Jun 11, 2021·Frontiers in Immunology·Anaïs RousseauxMarie Bodinier

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