PMID: 7523524Sep 30, 1994Paper

Human monoclonal anti-D secreting heterohybridomas from peripheral B lymphocytes expanded in the CD40 system

Journal of Immunological Methods
J M ThompsonD F McDonald

Abstract

Peripheral human B lymphocytes isolated from four immunised anti-D donors have been cultured in the CD40 system (Banchereau et al., 1991), prior to fusion to murine X63Ag8.653 plasmacytoma cells. High fusion efficiency was noted in all cases and immunoglobulin (IgG and IgM) was detected in nearly all heterohybrid containing wells. Only fusions using boosted donor lymphocytes generated specific anti-D secreting hybrids. A short period of culture of committed B cell precursors in the CD40 system before fusion appears to be both adequate and efficient in permitting generation of specific antibody-secreting hybrids.

References

Jan 1, 1991·Vox Sanguinis·M Lecointre-CoatmelecB Genetet
Oct 17, 1991·Nature·J Banchereau, F Rousset
Jul 1, 1985·Human Immunology·A DoyleB A Bradley
Jun 1, 1991·Transfusion Medicine·K ThompsonM Melamed

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