PMID: 9550326Apr 29, 1998Paper

Human monoclonal antibody with T-cell-like specificity recognizes MHC class I self-peptide presented by HLA-DR1 on activated cells

Tissue Antigens
A WölplT H Eiermann

Abstract

Alloreactive T cells recognize peptides presented in the binding groove of major histocompatibility complex molecules (MHCs), whereas B cells mainly recognize the MHCs independent of bound peptides. Here, we demonstrate that the human B-cell repertoire comprises B cells which can be stimulated during pregnancy to produce antibodies reacting with MHCs in a way similar to T cells. The human monoclonal antibody UL-5A1 recognizes DR1(DRA/DRB1*0101) molecules on lymphoblastoid cell lines only if they co-express HLA-A2 or if they have been loaded with HLA-A2-derived peptides. The effect of the HLA-A2 peptide 105-117 on UL-5A1 reactivity was specific, time and dose-dependent. Reactivity increased when naturally processed peptides were removed from DR1 molecules before the HLA-A2 peptide 105-117 was loaded. UL-5A1 reacted specifically with cells that had been activated. The results imply a role of activation of cells in peptide processing and/or loading.

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Citations

Dec 24, 2018·Molecular & Cellular Proteomics : MCP·Padma P NanawareLawrence J Stern
Sep 24, 2019·Antibodies·Lene Støkken HøydahlGeir Åge Løset
Mar 29, 2012·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Anja LehnhardtMarkus J Kemper
Mar 1, 2019·Molecular & Cellular Proteomics : MCP·Padma P NanawareLawrence J Stern

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