Human mononuclear phagocyte transglutaminase activity cross-links fibrin

Thrombosis Research
P R ConklingJ B Weinberg

Abstract

The physiologic function of the monocyte transglutaminases is not known. In this study, we detected Factor XIII A-subunit antigen and "tissue" transglutaminase antigen in human monocytes by polyacrylamide gel electrophoresis and immunoblotting techniques. Flow cytometric analysis demonstrated that 27% and 49% of the total Factor XIII antigen in monocytes and human peritoneal macrophages, respectively, are expressed on the surface of the cells. Monocytes maintained in culture for 8 days had a 4-fold increase in Factor XIIIa activity and a 3.2-fold increase in the amount of Factor XIII antigen/mg cell protein. However, there was no increase in the "tissue" transglutaminase activity or antigen levels in cultured monocytes. In addition, we identified a Factor XIII deficient individual who does not express Factor XIII activity or antigen in plasma, platelets, monocytes, lymphocytes or erythrocytes. Intact monocytes from normal donors were able to cross-link fibrin formed in the plasma from the Factor XIII deficient individual. This suggests that transglutaminase activity expressed by peripheral blood monocytes may play a physiologic role in cross-linking fibrin during blood clotting or inflammation.

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Citations

Jun 24, 1999·Thrombosis Research·L MuszbekZ Hevessy
Nov 24, 1999·International Journal of Biological Macromolecules·T ToidaR J Linhardt
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·David C SaneC S Greenberg
Mar 20, 2012·Thrombosis Research·Zsuzsa BagolyL Muszbek
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Nov 30, 2018·Journal of Thrombosis and Haemostasis : JTH·J L Mitchell, N J Mutch
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Related Concepts

Surface Antigens
Blood Platelets
SDS-PAGE
Erythrocytes
Factor Xiii Deficiency
Antithrombin I
Lymphoid Cells
Macrophage
Monocytes
Transglutaminases

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