Human multipotent adult progenitor cell-conditioned medium improves wound healing through modulating inflammation and angiogenesis in mice.

Stem Cell Research & Therapy
Parinaz AhangarA J Cowin

Abstract

Stem cell therapies have been widely investigated for their healing effects. However, the translation of these therapies has been hampered by the requirement to deliver live allogeneic or autologous cells directly to the wound in a clinical setting. Multipotent adult progenitor cells (MAPC® cells) are a subpopulation of bone marrow-derived adherent stem cells that secrete a wide range of factors known to accelerate the wound healing process. The aim of this study was to determine the impact of MAPC cells secretome on healing outcomes without the presence of MAPC cells. The effect of MAPC-conditioned medium (MAPC-CM) on the capacity of keratinocytes, fibroblasts and endothelial cells to migrate and proliferate was determined in vitro using scratch wound closure and WST1 assay, respectively. The effect of MAPC-CM on collagen deposition and angiogenesis was also assessed using in vitro methods. Additionally, two excisional wounds were created on the dorsal surface of mice (n = 8/group) and 100 μL of 20× MAPC-CM were intradermally injected to the wound margins. Wound tissues were collected at 3, 7 and 14 days post-wounding and stained with H&E for microscopic analysis. Immunohistochemistry was performed to investigate inflammation,...Continue Reading

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Citations

Sep 30, 2020·International Journal of Molecular Sciences·Parinaz AhangarAllison J Cowin
Mar 4, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Parinaz AhangarAllison J Cowin
Apr 4, 2021·International Journal of Molecular Sciences·Silvia Fernández-FrancosFrancisco J Vizoso
Aug 10, 2021·Frontiers in Cell and Developmental Biology·Trinidad Montero-VilchezSalvador Arias-Santiago

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Methods Mentioned

BETA
fluorescence microscopy

Software Mentioned

cellSense
GraphPad Prism
Incucyte zoom
ImageJ
Incucyte
CellSens dimension
Image Pro - Plus

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