Human multipotent mesenchymal stromal cells use galectin-1 to inhibit immune effector cells

Blood
Friederike GiesekeIngo Müller

Abstract

Human multipotent mesenchymal stromal cells (MSCs) suppress proliferation and alloreactivity of T cells. Several signaling molecules and enzymes contribute to this effect. We focused on carbohydrate-protein interactions and investigated whether lectins are involved in immune modulation by MSC. Gene expression profiling of MSCs revealed that one of the most important lectins in this setting, galectin-1, was highly expressed. Galectin-1 protein was detected intracellularly and on the cell surface of MSCs. In addition, galectin-1 was released into the cell culture supernatant by MSCs. To analyze the functional role of galectin-1, a stable knockdown of galectin-1 in MSCs with use of a retroviral transfection system was established. Galectin-1 knockdown in MSCs resulted in a significant loss of their immunomodulatory properties, compared with MSCs infected with nontargeting control sequences. The galectin-1 knockdown partially restored the proliferation of CD4(+) and CD8(+) T cells. By contrast, the effect of MSCs on nonalloreactive natural killer (NK) cells was unaffected by down-regulation of galectin-1 expression. Furthermore, MSC-derived galectin-1 significantly modulated the release of cytokines involved in graft-versus-host di...Continue Reading

References

Dec 14, 1995·Nature·N L PerilloL G Baum
Feb 2, 2002·Experimental Hematology·Amelia BartholomewRonald Hoffman
Mar 23, 2002·Science·Thijn R BrummelkampReuven Agami
Jun 20, 2002·Trends in Immunology·Gabriel A RabinovichStefano Iacobelli
Sep 24, 2002·Glycobiology·Joseph D Hernandez, Linda G Baum
Sep 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julius A PotianPranela Rameshwar
Nov 18, 2003·The Journal of Biological Chemistry·Jiale He, Linda G Baum
Dec 31, 2003·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Linda G BaumGayle Cocita Baldwin
Oct 21, 2004·Blood·Sudeepta Aggarwal, Mark F Pittenger
Dec 14, 2004·European Journal of Immunology·Agustina Tri EndhartiHaruhiko Suzuki
Aug 16, 2005·Stem Cells·Panagiota A SotiropoulouMichael Papamichail
Apr 25, 2006·Experimental Cell Research·Ida Rasmusson
Jun 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jennifer A FulcherBenhur Lee
Jul 15, 2006·Glycobiology·Isabelle CambyRobert Kiss
Nov 18, 2006·Blood·Marina I GarínRobert I Lechler
Jun 27, 2007·Blood·Victor L J L ThijssenArjan W Griffioen
Aug 1, 2007·Blood·Alma J Nauta, Willem E Fibbe
Aug 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Przemyslaw JuszczynskiMargaret A Shipp
Nov 21, 2007·Nature Medicine·Sandra M BloisPetra C Arck
May 13, 2008·Lancet·Katarina Le BlancUNKNOWN Developmental Committee of the European Group for Blood and Marrow Transplantation
Jun 14, 2008·Expert Reviews in Molecular Medicine·Ri-Yao YangFu-Tong Liu
Nov 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Hernan D KopcowJack L Strominger
Dec 9, 2008·Current Opinion in Organ Transplantation·Ingo MüllerFrancesco Dazzi
Apr 15, 2009·Nature Reviews. Immunology·Gabriel A Rabinovich, Marta A Toscano

❮ Previous
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Citations

Dec 21, 2011·International Journal of Hematology·Lei HaoZhongmin Zou
Mar 31, 2011·Nature Reviews. Clinical Oncology·Claudio TripodoStefano A Pileri
Oct 15, 2011·Tissue Engineering. Part B, Reviews·Joyce DoornJan de Boer
Apr 9, 2013·Tissue Engineering. Part C, Methods·Anna OtteRalf Hass
Sep 18, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nicole CarpeFeige Kaplan
Jul 19, 2011·Journal of Biomedical Science·Pei-Min ChenB-Linju Yen
Sep 5, 2012·Cell Communication and Signaling : CCS·Ralf Hass, Anna Otte
Aug 25, 2011·Stem Cell Research & Therapy·Michelle M DuffyMatthew D Griffin
Jun 20, 2012·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Siddaraju V Boregowda, Donald G Phinney
Nov 5, 2013·Cell Death and Differentiation·S MaY Wang
Jan 28, 2014·Bone Marrow Transplantation·H KaipeO Ringdén
Jul 16, 2014·BioMed Research International·Rodrigo Haddad, Felipe Saldanha-Araujo
Dec 12, 2012·Current Opinion in Organ Transplantation·James L ReadingTimothy I M Tree
Feb 9, 2016·Nature Reviews. Nephrology·Federica CasiraghiGiuseppe Remuzzi
Sep 10, 2015·FEBS Letters·Ada G BlidnerGabriel A Rabinovich
Jul 3, 2013·European Journal of Immunology·Friederike GiesekeIngo Müller
Dec 23, 2015·Transfusion Medicine Reviews·Magali J FontaineMark F Pittenger
Jun 17, 2011·Journal of Cellular Biochemistry·Eva Mezey

❮ Previous
Next ❯

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