Human muscle sarcoplasmic reticulum function during submaximal exercise in normoxia and hypoxia

Journal of Applied Physiology
T A DuhamelJ Ouyang

Abstract

In this study, the response of the sarcoplasmic reticulum (SR) to prolonged exercise, performed in normoxia (inspired O(2) fraction = 0.21) and hypoxia (inspired O(2) fraction = 0.14) was studied in homogenates prepared from the vastus lateralis muscle in 10 untrained men (peak O(2) consumption = 3.09 +/- 0.25 l/min). In normoxia, performed at 48 +/- 2.2% peak O(2) consumption, maximal Ca(2+)-dependent ATPase activity was reduced by approximately 25% at 30 min of exercise compared with rest (168 +/- 10 vs. 126 +/- 8 micromol.g protein(-1) x min(-1)), with no further reductions observed at 90 min (129 +/- 6 micromol x g protein(-1) x min(-1)). No changes were observed in the Hill coefficient or in the Ca(2+) concentration at half-maximal activity. The reduction in maximal Ca(2+)-dependent ATPase activity at 30 min of exercise was accompanied by oxalate-dependent reductions (P < 0.05) in Ca(2+) uptake by approximately 20% (370 +/- 22 vs. 298 +/- 25 micromol x g protein(-1) x min(-1)). Ca(2+) release, induced by 4-chloro-m-cresol and assessed into fast and slow phases, was decreased (P < 0.05) by approximately 16 and approximately 32%, respectively, by 90 min of exercise. No differences were found between normoxia and hypoxia for ...Continue Reading

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Citations

Jan 18, 2007·European Journal of Applied Physiology·Satoshi MatsunagaMasanobu Wada
Dec 22, 2005·Pflügers Archiv : European journal of physiology·Takaaki MishimaMasanobu Wada
Nov 18, 2011·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Howard J GreenSusan Tupling
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Jun 18, 2011·American Journal of Physical Medicine & Rehabilitation·Howard J GreenJing Ouyang
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Aug 11, 2005·Canadian Journal of Physiology and Pharmacology·T A DuhamelJ Ouyang
Jan 31, 2008·American Journal of Physiology. Endocrinology and Metabolism·H J GreenJ Ouyang
May 30, 2008·American Journal of Physiology. Cell Physiology·H J GreenJ Ouyang
Jul 25, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Howard J GreenJing Ouyang
May 13, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·T A DuhamelH J Green
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