Human myosin VIIa is a very slow processive motor protein on various cellular actin structures.

The Journal of Biological Chemistry
Osamu SatoMitsuo Ikebe

Abstract

Human myosin VIIa (MYO7A) is an actin-linked motor protein associated with human Usher syndrome (USH) type 1B, which causes human congenital hearing and visual loss. Although it has been thought that the role of human myosin VIIa is critical for USH1 protein tethering with actin and transportation along actin bundles in inner-ear hair cells, myosin VIIa's motor function remains unclear. Here, we studied the motor function of the tail-truncated human myosin VIIa dimer (HM7AΔTail/LZ) at the single-molecule level. We found that the HM7AΔTail/LZ moves processively on single actin filaments with a step size of 35 nm. Dwell-time distribution analysis indicated an average waiting time of 3.4 s, yielding ∼0.3 s-1 for the mechanical turnover rate; hence, the velocity of HM7AΔTail/LZ was extremely slow, at 11 nm·s-1 We also examined HM7AΔTail/LZ movement on various actin structures in demembranated cells. HM7AΔTail/LZ showed unidirectional movement on actin structures at cell edges, such as lamellipodia and filopodia. However, HM7AΔTail/LZ frequently missed steps on actin tracks and exhibited bidirectional movement at stress fibers, which was not observed with tail-truncated myosin Va. These results suggest that the movement of the human...Continue Reading

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Citations

Jan 8, 2019·Climacteric : the Journal of the International Menopause Society·H DiaoL Mao
Aug 28, 2020·Science Advances·Giusy A CapraraAnthony W Peng
Nov 29, 2020·Developmental Biology·Jennifer L SalleeDaniel P Kiehart
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Feb 23, 2020·Biochemical and Biophysical Research Communications·Xianan QinHyokeun Park
Apr 24, 2021·Journal of Analytical & Pharmaceutical Research·Annapurna Kuppa, Yuri V Sergeev

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