Nov 8, 2018

Human Nerve-on-a-Chip. Engineering 3D functional human peripheral nerve in vitro

BioRxiv : the Preprint Server for Biology
Anup Dutt SharmaMichael J Moore

Abstract

Development of "organ-on-a-chip" systems in the space of the nervous system is lagging because of lack of availability of human neuronal & glial cells and the structural complexity of the nervous system. Furthermore, a sizeable challenge in the drug development pipeline and an often-cited reason for failure in a vast number of clinical trials conducted for neuropathological ailments is the lack of translation from animal models. A preclinical in vitro model capable of mimicking the function and structure of the human nervous system is well desired. We present an in vitro biomimetic model of all-human peripheral nerve tissue capable of showing a robust neurite outgrowth (~5mm), myelination of human induced pluripotent stem cells (iPSCs)-derived neurons by primary human Schwann cells and evaluation of nerve conduction velocity (NCV), previously unrealized for any human cell-based in vitro system. This Human Nerve-on-a-chip (HNoaC) system is the first microphysiological system of human peripheral nerve which can be used for evaluating electrophysiological and histological metrics, which are gold-standard assessment techniques previously only possible with in vivo studies.

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Mentioned in this Paper

Drug Development
Pluripotent Stem Cells
Three-dimensional
Neurite Outgrowth
Electrophysiologic Evaluation of Single or Dual Chamber Pacing Cardioverter-defibrillator (Includes Defibrillation Threshold Evaluation, Induction of Arrhythmia, Evaluation of Sensing and Pacing for Arrhythmia Termination, and Programming or Reprogramming
Entire Nervous System
Neurons
Human Induced Pluripotent Stem Cells
Neuroglia
Protein Biosynthesis

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