Human neural stem cells improve sensorimotor deficits in the adult rat brain with experimental focal ischemia

Brain Research
Kon ChuJae-Kyu Roh

Abstract

Ischemic stroke is caused by the interruption of cerebral blood flow that leads to brain damage with long-term sensorimotor deficits. Stem cell transplantation may recover functional deficit by replacing damaged brain. In this study, we attempted to test whether the human neural stem cells (NSCs) can improve the outcome in the rat brain with intravenous injection and also determine the migration, differentiation and the long-term viabilities of human NSCs in the rat brain. Focal cerebral ischemia was induced by intraluminal thread occlusion of middle cerebral artery (MCA). One day after surgery, the rats were randomly divided into two groups: NSCs-ischemia vs. Ischemia-only. Human NSCs infected with retroviral vector encoding beta galactosidase were intravenously injected in NSCs-ischemia group (5 x 10(6) cells) and the same amount of saline was injected in Ischemia-only group for control. The animals were evaluated for 4 weeks using turning in an alley (TIA) test, modified limb placing test (MLPT) and rotarod test. Transplanted cells were detected by X gal cytohistochemistry or beta gal immunohistochemistry with double labeling of other cell markers. The NSCs-ischemia group showed better performance on TIA test at 2 weeks, and...Continue Reading

References

Mar 11, 2000·The European Journal of Neuroscience·C Vicario-AbejónR D McKay
May 3, 2000·The European Journal of Neuroscience·J M AuerbachR D McKay
Nov 9, 2000·Proceedings of the National Academy of Sciences of the United States of America·K S AboodyE Y Snyder
Feb 7, 2001·Science·E M UllianB A Barres
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·K JinD A Greenberg
Aug 4, 2001·Stroke; a Journal of Cerebral Circulation·Y YagitaM Matsumoto
Mar 5, 2002·Nature·Henriette van PraagFred H Gage
May 3, 2002·Nature·Hongjun SongFred H Gage
Sep 3, 2002·Nature Medicine·Evan Y Snyder, Kook I Park
Sep 3, 2002·Annals of Neurology·Sean I SavitzLouis R Caplan
Sep 7, 2002·Stroke; a Journal of Cerebral Circulation·Michel ModoHelen Hodges
Nov 26, 2002·Annals of Neurology·Jack M ParentDonna M Ferriero
Dec 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ulrica EnglundMerab Kokaia
Feb 21, 2003·Current Opinion in Neurobiology·Zaal Kokaia, Olle Lindvall
Jul 18, 2003·Journal of Neuroscience Research·A E WillingP R Sanberg
Jul 26, 2003·Stroke; a Journal of Cerebral Circulation·Sang-Wuk JeongJae-Kyu Roh
Aug 9, 2003·Stroke; a Journal of Cerebral Circulation·Eun-Chol SongByung-Woo Yoon

❮ Previous
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Citations

Mar 9, 2007·Neuroradiology·Letterio S Politi
Sep 22, 2007·European Journal of Nuclear Medicine and Molecular Imaging·Do Won HwangDong Soo Lee
Jun 24, 2011·European Journal of Nuclear Medicine and Molecular Imaging·Jiachuan WangHong Zhang
Sep 29, 2007·Cell and Tissue Research·Rike ZietlowAnne E Rosser
Feb 11, 2012·Cell and Tissue Research·Chiara CossettiStefano Pluchino
Apr 7, 2007·Bulletin of Experimental Biology and Medicine·I V ViktorovV P Chekhonin
May 20, 2006·Cellular and Molecular Neurobiology·Konstantin-Alexander Hossmann
May 13, 2006·Cellular and Molecular Neurobiology·Sona BálentováEva Misúrová
Jul 28, 2012·Nature Neuroscience·Zaal KokaiaOlle Lindvall
Nov 13, 2010·Age and Ageing·Soma BanerjeeJeremy Chataway
Dec 12, 2007·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Manho KimSeung U Kim
Jan 28, 2009·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Pengbo ZhangQianyan Kang
Sep 30, 2010·PloS One·Pedro Ramos-CabrerMathias Hoehn
Oct 24, 2012·Vascular Health and Risk Management·Soma BanerjeeJeremy Chataway
Mar 18, 2008·Neurosurgical Focus·Raphael GuzmanGary K Steinberg
Aug 27, 2005·Journal of Neurosurgery·Roy A E Bakay
Oct 3, 2007·Regenerative Medicine·Robert H Miller, Lianhua Bai
May 18, 2011·Cellular and Molecular Life Sciences : CMLS·Lidia De Filippis, Domenico Delia
Nov 5, 2014·Neural Regeneration Research·Wenxin HuoJiajun Chen
Oct 17, 2013·Stroke; a Journal of Cerebral Circulation·Hiroto IshikawaCesar V Borlongan
Feb 11, 2016·International Journal of Molecular Sciences·Nunzio IraciStefano Pluchino
Dec 19, 2012·Revue neurologique·L PellegriniP Pisano
Apr 2, 2011·Neurochemistry International·Dheeraj Kalladka, Keith W Muir
Jun 18, 2010·Neurobiology of Disease·Thorsten R DoeppnerMathias Bähr
Oct 14, 2009·Neurobiology of Disease·Tonya M BlissGary K Steinberg
Nov 17, 2009·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Wanfu WuWenqin Cai
Jul 25, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Thorsten R DoeppnerMathias Bähr
Nov 6, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Lian LiMichael Chopp
Jun 10, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Vladimer DarsaliaZaal Kokaia
Jul 29, 2008·Journal of the Neurological Sciences·Miroslaw JanowskiBarbara Lukomska

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