Human neuroblastoma cells transfected with tyrosine hydroxylase gain increased resistance to methylmercury-induced cell death

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Thaís PosserJeferson Luis Franco

Abstract

In a previous study we demonstrated that human neuroblastoma SH-SY5Y cells transfected with human tyrosine hydroxylase isoform 1 (SH+TH cells) were substantially more resistant to cell death induced by pro-oxidants than wild type SH-SY5Y cells (SH cells). In the present communication we used methylmercury as a model of cell stress in order to test whether SH+TH cells would behave in a similar manner in response to this stressor. Incubation with methylmercury (0.1-3 microM) for 24h caused a significant reduction in cell viability and increased apoptotic markers in both cell types. However, the effects were significantly reduced in the SH+TH cells when compared to the SH cells. Activation of p38(MAPK) was also reduced in the SH+TH compared to the SH cells after methylmercury exposure. Since p38(MAPK) is known to participate in signal transduction pathways during cell stress, our data suggest that SH+TH cells develop an increased resistance to environmental stress caused by neurotoxins such as methylmercury. In conclusion our results show that insertion of the human TH gene in cells that originally do not express this protein leads to alterations in cell homeostasis and triggers defense mechanisms against pro-oxidative insults.

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Citations

May 20, 2014·Environmental Toxicology and Pharmacology·Mitsuya ShiraishiAtsushi Miyamoto
Jul 11, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Isabel Cordero-HerreraSonia Ramos
Feb 6, 2017·Frontiers in Neuroscience·Natascia GuidaLuigi Formisano
Nov 14, 2019·Toxics·Megan Culbreth, Michael Aschner

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