Human neutralizing antibodies elicited by SARS-CoV-2 infection.

Nature
Bin JuLinqi Zhang

Abstract

The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) presents a global health emergency that is in urgent need of intervention1-3. The entry of SARS-CoV-2 into its target cells depends on binding between the receptor-binding domain (RBD) of the viral spike protein and its cellular receptor, angiotensin-converting enzyme 2 (ACE2)2,4-6. Here we report the isolation and characterization of 206 RBD-specific monoclonal antibodies derived from single B cells from 8 individuals infected with SARS-CoV-2. We identified antibodies that potently neutralize SARS-CoV-2; this activity correlates with competition with ACE2 for binding to RBD. Unexpectedly, the anti-SARS-CoV-2 antibodies and the infected plasma did not cross-react with the RBDs of SARS-CoV or Middle East respiratory syndrome-related coronavirus (MERS-CoV), although there was substantial plasma cross-reactivity to their trimeric spike proteins. Analysis of the crystal structure of RBD-bound antibody revealed that steric hindrance inhibits viral engagement with ACE2, thereby blocking viral entry. These findings suggest that anti-RBD antibodies are largely viral-species-specific inhibitors. The antibodies identifi...Continue Reading

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Citations

Jul 22, 2020·The Journal of Experimental Medicine·Fabian SchmidtPaul D Bieniasz
Jun 28, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wafaa B AlsoussiAli H Ellebedy
Aug 29, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tyler E MillerJochen K Lennerz
Sep 18, 2020·Expert Opinion on Therapeutic Targets·Shibo JiangLanying Du
Sep 24, 2020·Reviews in Medical Virology·Eamon O MurchuPatricia Harrington
Sep 12, 2020·Bioanalysis·Caroline Becker
Aug 6, 2020·Frontiers in Public Health·Waquar AhsanShahnaz Sultana
Aug 19, 2020·Nature Medicine·Adam G LaingAdrian C Hayday
Aug 28, 2020·Signal Transduction and Targeted Therapy·Francesca FagianiCristina Lanni
Jul 6, 2020·Nature·Gary R Whittaker, Susan Daniel
Aug 2, 2020·Nature Structural & Molecular Biology·Daming ZhouKuan-Ying A Huang
Sep 24, 2020·Signal Transduction and Targeted Therapy·Fei YuShibo Jiang
Sep 20, 2020·Science Advances·T KondoH Murakami
Aug 2, 2020·Nature Structural & Molecular Biology·Xiaoli XiongJohn A G Briggs
Sep 4, 2020·Signal Transduction and Targeted Therapy·Xiangyu ChenLilin Ye
Sep 6, 2020·Nature Communications·Leo HankeGerald M McInerney
Sep 11, 2020·Nature Microbiology·Wen Shi LeeBrandon J DeKosky
Jun 27, 2020·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·Focke ZiemssenMarius Ueffing
Sep 12, 2020·Nature Reviews. Microbiology·Emmanuel MargolinEdward P Rybicki
Oct 7, 2020·Journal of Cellular Physiology·Abdolreza Esmaeilzadeh, Reza Elahi
Jul 22, 2020·Journal of Medical Virology·Yunfei WangCunbao Liu

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
surface plasmon resonance
gel filtration
FACS
PCR
transfection
Protein Assay
chip
Assay

Software Mentioned

Clustal W
IMGT
BioEdit
PHENIX
- QUEST
PHASER
MEGA X
FlowJo
GraphPad
GraphPad Prism

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