PMID: 2113768Jun 1, 1990Paper

Human neutrophil stimulation by influenza virus: relationship of cytoplasmic pH changes to cell activation

The American Journal of Physiology
K L HartshornA I Tauber

Abstract

We have previously demonstrated that influenza A virus (IAV) stimulates the human neutrophil through phospholipase C activation. With the use of the fluorescent indicator 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF), cytoplasmic acidification and subsequent alkalinization are shown to accompany this activation. These responses are not inhibited by pertussis toxin (PT). The alkalinization is mediated largely *but not entirely) by the Na(+)-H+ antiporter and is not initiated, or modulated, by the IAV-induced cytosolic Ca2+ (Cai2+) rise. Rather, protein kinase C (PKC) is likely the mediator of cell alkalinization, based on studies using the PKC inhibitor 1-(5-isoquinolinesulfonyl)-2-methylpiperazine (H-7). The acidification can be dissociated from the alkalinization response, which is also independent of Cai2+ fluxes and of PKC. Both pHi responses can be dissociated from the respiratory burst. Cytosolic alkalinization and acidification seem to reflect two independently mediated responses of the activated neutrophil, the former resulting ultimately from phospholipase activation and the latter from other activities that are not yet fully characterized.

References

Feb 1, 1988·Journal of Leukocyte Biology·J WrightA I Tauber
Sep 1, 1985·The Journal of Clinical Investigation·L Simchowitz
Mar 1, 1986·The Journal of Clinical Investigation·J WrightA I Tauber
Mar 1, 1988·Reviews of Infectious Diseases·J S Abramson, E L Mills

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Citations

Dec 31, 1997·The American Journal of Physiology·W E CroweJ M Russell
Nov 28, 2000·Cell Motility and the Cytoskeleton·B W BernsteinJ R Bamburg
Feb 25, 1998·Journal of Cardiovascular Pharmacology·S N WuT L Hwang
Jul 1, 2008·Expert Review of Clinical Immunology·Mitchell R WhiteKevan L Hartshorn
Jan 1, 1993·Pacing and Clinical Electrophysiology : PACE·E D Engelstein, B B Lerman
May 5, 1999·Journal of Cardiovascular Pharmacology·R F BoschL Seipel
Oct 26, 2005·Circulation Journal : Official Journal of the Japanese Circulation Society·Hiroshi KatohKunio Shirato
Jun 29, 1992·Biochimica Et Biophysica Acta·M G RaeA K Dutta-Roy

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