PMID: 9646841Jul 1, 1998Paper

Human neutrophils express the prostaglandin G/H synthase 2 gene when stimulated with bacterial lipopolysaccharide

Clinical Immunology and Immunopathology
M B FasanoC McCall

Abstract

Human blood neutrophils (PMN) rapidly release arachidonic acid (AA) from cellular phospholipids when stimulated in vitro with a variety of inflammatory agonists. Free AA is then metabolized via 5'-lipoxygenase to produce bioactive mediators such as leukotriene B4 and 5-hydroxyeicosatetraenoate. Arachidonic acid can also be metabolized via the cyclooxygenase or prostaglandin G/H synthase (PGHS) pathway to form prostaglandins and thromboxane. We show here that human blood PMN express the PGHS 2 gene when stimulated with bacterial lipopolysaccharide (LPS). PGHS 2 mRNA increases within 30 min after LPS stimulation and PGHS 2 immunoreactive protein is detectable by 5 h. Although PGHS 1 mRNA is detectable in PMN, no immunoreactive protein is observed in either resting or LPS-stimulated cells. Following stimulation with LPS and expression of PGHS 2, PMN increase secretion of prostaglandin E2. This phenotypic change in PMN could be an important mechanism for regulating inflammation.

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Citations

Dec 13, 2000·Neuroscience·N M Perkins, D J Tracey
Jan 5, 2002·Clinical and Diagnostic Laboratory Immunology·Jörg EberhardYahya Açil
Mar 17, 2005·Journal of Cell Science·Jean-Sébastien CadieuxMarc Pouliot
Jun 12, 2012·Pharmacology, Biochemistry, and Behavior·Maria Cláudia G Oliveira-FusaroCláudia Herrera Tambeli
Aug 23, 2016·JCI Insight·Liudmila L MazaleuskayaGarret A FitzGerald
Aug 20, 2016·Journal of Lipid Research·Philippe Pierre RobichaudMarc E Surette
Oct 22, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Julie S LefebvrePierre Borgeat
Mar 20, 2003·Microscopy Research and Technique·Wolfgang NackenClaus Kerkhoff
Oct 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marc PouliotPierre Borgeat
May 16, 2002·International Immunopharmacology·Bianca Rocca, Garret A FitzGerald

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