PMID: 9657514Jul 10, 1998Paper

Human occult loiasis: field evaluation of a nested polymerase chain reaction assay for the detection of occult infection

Tropical Medicine & International Health : TM & IH
F S ToureT G Egwang

Abstract

A nested polymerase chain reaction (nested PCR) assay, targeted on the repeat 3 region (15r3) of the gene coding for a Loa loa 15 kD polyprotein, was developed to detect L. loa infection. The assay has a sensitivity of 95% and is 100% specific with regard to sympatric filarial parasites: Mansonella perstans, Onchocerca volvulus and Wuchereria bancrofti. In this field study in a mixed filarial (L. loa and M. perstans) endemic region of Gabon, 157 L. loa amicrofilaraemic blood samples (AMF; diagnosed by leucoconcentration followed by standard microscopic examination) from the residents from four villages were screened by the 15r3-nested PCR assay. The assay detected 106 occult infected subjects among the 157 AMF individuals (68%), including 59 of 87 adults (68%) and 47 of 70 children (67%). In each village the prevalence of occult infection was, respectively, 38%, 52%, 79% and 80% for Moyabi, Djoutou, N'djokaye and Okoumbi. The annual transmission potential (ATP) of loiasis has been estimated to be 250 infective larvae (L 3) per man per year for Moyabi and Djoutou, 1800 for N'djokaye and 433000 L3/man/year for Okoumbi. This implies a correlation between occult infection of loiasis and the intensity of transmission. By contrast, t...Continue Reading

References

Jun 1, 1987·Annals of Tropical Medicine and Parasitology·M Van HoegaerdenB Chabaud
Sep 1, 1995·Experimental Parasitology·P M AjuhT G Egwang
Nov 1, 1994·Transactions of the Royal Society of Tropical Medicine and Hygiene·S ChanteauS A Williams
Sep 1, 1994·The American Journal of Tropical Medicine and Hygiene·M R LizotteS A Williams
Apr 1, 1996·The American Journal of Tropical Medicine and Hygiene·M ZhongS A Williams
Mar 1, 1996·Transactions of the Royal Society of Tropical Medicine and Hygiene·J P AkueE Devaney
Mar 1, 1996·Transactions of the Royal Society of Tropical Medicine and Hygiene·L NicolasD R Mercer
Jul 1, 1996·Transactions of the Royal Society of Tropical Medicine and Hygiene·S A WilliamsJ P Moulia-Pelat
Jul 1, 1997·Experimental Parasitology·F S TouréT G Egwang
Jun 12, 1998·Tropical Medicine & International Health : TM & IH·F S TouréG Wahl
Jan 1, 1994·Parasitology Today·T B NutmanD D Kostyu
Oct 1, 1988·Parasitology Today·M Pinder
Nov 1, 1993·Parasitology Today·L A McReynoldsM E Selkirk

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Citations

May 10, 2006·Transactions of the Royal Society of Tropical Medicine and Hygiene·Olivia VeitChristoph Hatz
Dec 18, 2013·The Lancet Infectious Diseases·Wolfram Gottfried Metzger, Benjamin Mordmüller
May 30, 2008·Journal of Clinical Microbiology·Peter D BurbeloThomas B Nutman
Apr 10, 2010·Parasites & Vectors·Puteri Azaziah Megat Abd RaniRebecca J Traub
Sep 14, 2011·PLoS Neglected Tropical Diseases·Doran L FinkThomas B Nutman
Sep 13, 2011·Enfermedades infecciosas y microbiología clínica·Maribel JiménezTeresa Gárate
Jul 15, 2015·Tropical Medicine and Health·Tetsuro KobayashiYasuyuki Kato
Jan 26, 2008·Journal of Travel Medicine·Masahide YoshikawaYukifumi Nawa
Nov 3, 2005·Tropical Medicine & International Health : TM & IH·Donatien MavoungouElie Mavoungou
Feb 24, 2007·Annals of Tropical Medicine and Parasitology·I TakougangM Ntep
Jan 29, 2000·Health Affairs·U E Reinhardt
May 27, 2020·PLoS Neglected Tropical Diseases·Federico GobbiZeno Bisoffi

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