PMID: 8950502Nov 1, 1996Paper

Human ocular melanocytes and retinal pigment epithelial cells differ in their melanogenic properties in vivo and in vitro

Current Eye Research
L Smith-ThomasS MacNeil

Abstract

The vertebrate eye contains both melanocytes and retinal pigment epithelial (RPE) cells. Little is known of the pigmentary behaviour of these embryologically dissimilar cells. The aim of this study was to examine aspects of the pigmentary properties of both cell types in vitro and ex vivo to learn more of the function of these cells. Sections of normal adult human eye were stained for tyrosinase related protein 1(TRP1), and cultures of RPE cells and choroidal melanocytes were examined immunocytochemically for TRP1 and 2 and enzymatically for tyrosinase activity (by assaying dopa oxidase activity). Over half of the choroidal melanocytes expressed TRP1 ex vivo; in contrast, a very small percentage of RPE cells were TRP1 positive. In vitro, passage 1 to 3 ocular melanocytes expressed TRP1 and TRP2 and had tyrosinase activity, which was influenced by the choice of substrate on which the cells were grown. Tyrosinase activity was highest when cells were grown on fibronectin and plastic, intermediate on laminin and lowest on vitreous extracellular matrix (ECM) containing pigment to which they attached and spread out poorly. In contrast, passage 3 RPE cells (which were unpigmented) showed little evidence of tyrosinase activity in short...Continue Reading

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Citations

Feb 1, 1997·Pigment Cell Research·S J HedleyS MacNeil
Feb 27, 2009·American Journal of Ophthalmology·Simone KellnerKlaus Ruether
Apr 23, 2004·The Journal of Investigative Dermatology·Richard J ElliottJohn W Haycock
Mar 19, 2008·Photochemistry and Photobiology·Dan-Ning HuTadeusz Sarna
Jan 15, 2011·Pigment Cell & Melanoma Research·Claudia ArutaValeria Marigo
Jan 25, 2012·Acta Ophthalmologica·Raimondo ForteEric H Souied
Aug 26, 2014·Experimental Eye Research·Michael E Boulton
Apr 4, 2006·Experimental Eye Research·Ulrich SchraermeyerKarl-Ulrich Bartz-Schmidt
Oct 9, 2015·Journal of Ophthalmology·Lara PasovicTor P Utheim
Jan 4, 2005·Ophthalmic Research·Rangaprasad Sarangarajan, Shireesh P Apte
Mar 31, 2004·Experimental Eye Research·L Smith-ThomasS MacNeil
Jan 23, 2003·International Journal of Toxicology·Pierrette Dayhaw-Barker
Jan 23, 2003·International Journal of Toxicology·Dan-Ning HuJoan E Roberts
Apr 27, 2007·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Monika Valtink, Katrin Engelmann
Feb 24, 2007·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Sylvie JulienUlrich Schraermeyer
Sep 8, 2012·Japanese Journal of Ophthalmology·Takeshi NakaoKohji Nishida
Aug 2, 2018·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Sally M YacoutElizabeth R Gaillard
Nov 21, 2000·The International Journal of Biochemistry & Cell Biology·K J Rodgers, R T Dean

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