PMID: 9185703Jun 11, 1997Paper

Human pancreatic cancer cells (MPanc-96) recognized by autologous tumor-infiltrating lymphocytes after in vitro as well as in vivo tumor expansion

International Journal of Cancer. Journal International Du Cancer
M PeiperT J Eberlein

Abstract

A human tumor line designated MPanc-96 has been established from a poorly differentiated primary pancreatic adenocarcinoma. MPanc-96 has a doubling time of 27 hr and grows as a confluent monolayer in various culture media. Cytogenetic analysis of in vitro-cultured tumor cells revealed a large number of clonal chromosomal aberrations, confirming their neoplastic origin. MPanc-96 grows in SCID mice when injected s.c. Xenografts established from the tumor line had a similar histology as the primary tumor. Tumor-infiltrating lymphocytes (TILs) were isolated from the primary tumor, and cytotoxic T lymphocytes (CTLs) were generated after activation on immobilized anti-CD3 monoclonal antibody (MAb) for 48 hr, expansion in low-dose IL-2 and repeated stimulation with irradiated MPanc-96 tumor cells. The generated CTLs lysed fresh autologous tumor cells as well as in vitro and in vivo expanded tumor cells from passages 9-53, suggesting that one or more tumor-associated antigens (TAAs) are stably expressed. CTLs lysed tumor cells in an HLA-class I-restricted fashion but showed no significant cytotoxicity against autologous fibroblasts, several allogeneic pancreatic cancer cell lines or K562. Our findings may be significant for the design ...Continue Reading

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Citations

Jan 11, 2013·Molecular Cancer Therapeutics·Thiruvengadam ArumugamCraig D Logsdon
Jan 5, 2001·Expert Opinion on Investigational Drugs·E S FerdinandiG Ely
Jul 23, 2005·Neoplasia : an International Journal for Oncology Research·Murali D BashyamJonathan R Pollack
Feb 24, 2015·Magnetic Resonance Imaging·Rossana RitelliPasquina Marzola
Apr 12, 2002·International Journal of Cancer. Journal International Du Cancer·Matthias PeiperJakob Robert Izbicki
Jul 2, 2011·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·L I ParteckeA Stier
Dec 21, 2006·Journal of the National Cancer Institute·Thiruvengadam ArumugamCraig D Logsdon
Dec 26, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ainhoa Perez-DiezFrancesco M Marincola
May 19, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Xinping FuXiaoliu Zhang

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