PMID: 9421083Jul 1, 1997Paper

Human papillomavirus distribution in cervical tissues of different morphology as determined by hybrid capture assay and PCR

International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists
I NindlA Schneider

Abstract

Distribution of various types of genital human Papillomavirus (HPV) in smears from histologically classified cervical lesions was determined by hybrid capture assay (HCA) and was compared with a polymerase chain reaction (PCR) system using general primers (GP) in first and type specific primers (TS) in a second step. The overall agreement of high-risk HPV by HCA and the more sensitive GP/TS PCR was 80.6% (204 of 253, kappa value 0.6). Human Papillomavirus frequency by GP/TS PCR was 14-20% higher compared with HCA (p = 0.02-0.004) independent of morphology. Only one sample was positive by HCA and negative by GP/TS PCR. A significantly higher frequency was found using HCA and GP/TS PCR in smears from histologically proven cervical intraepithelial lesions (CIN) II/III compared with CIN I, tissues with minimal changes (metaplasia, cervicitis, or lack of glycogenization), or normal morphology (61% and 81% vs 8-15% and 24-34%, p < or = 0.001). Semi-quantitative estimate of HPV DNA copies by GP-PCR coincided with estimated virus load by quantitative HCA and was significantly higher in patients with CIN II/III compared with CIN I (p < 0.001). Thus, the GP-PCR may be used to monitor the amount of HPV DNA copies in clinical samples. A di...Continue Reading

Citations

May 20, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Thomas JohnsonAnders Fomsgaard
Oct 8, 2011·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Luisa Benerini GattaAnna Benetti
Mar 20, 2012·Critical Reviews in Oncology/hematology·F HintenJ A de Hullu
Dec 10, 1998·The American Journal of Pathology·C P Crum
Nov 20, 1998·American Journal of Obstetrics and Gynecology·D M ZahmA Schneider
Jul 2, 1999·International Journal of Cancer. Journal International Du Cancer·I NindlM Dürst
Dec 23, 2006·Nature Reviews. Cancer·Ciaran B J WoodmanLawrence S Young
Jul 17, 2001·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·P BirnerG Oberhuber

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