Human papillomavirus load measured by Linear Array correlates with quantitative PCR in cervical cytology specimens.

Journal of Clinical Microbiology
N WentzensenSophia S Wang

Abstract

Carcinogenic human papillomavirus (HPV) infections are necessary causes of most anogenital cancers. Viral load has been proposed as a marker for progression to cancer precursors but has been confirmed only for HPV16. Challenges in studying viral load are related to the lack of validated assays for a large number of genotypes. We compared viral load measured by Linear Array (LA) HPV genotyping with the gold standard, quantitative PCR (Q-PCR). LA genotyping and Q-PCR were performed in 143 cytology specimens from women referred to colposcopy. LA signal strength was measured by densitometry. Correlation coefficients and receiver operating characteristic (ROC) analyses were used to evaluate analytical and clinical performance. We observed a moderate to strong correlation between the two quantitative viral load measurements, ranging from an R value of 0.61 for HPV31 to an R value of 0.86 for HPV52. We also observed agreement between visual LA signal strength evaluation and Q-PCR. Both quantifications agreed on the disease stages with highest viral load, which varied by type (cervical intraepithelial neoplasia grade 2 [CIN2] for HPV52, CIN3 for HPV16 and HPV33, and cancer for HPV18 and HPV31). The area under the curve (AUC) for HPV16 ...Continue Reading

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Citations

Dec 1, 2012·Journal of Clinical Microbiology·Jill KoshiolNicolas Wentzensen
Feb 1, 2014·Sexually Transmitted Infections·Mary K GrabowskiAaron A R Tobian
Nov 26, 2015·The Journal of Infectious Diseases·Mary K GrabowskiAaron A R Tobian
Oct 19, 2013·International Journal of Cancer. Journal International Du Cancer·Rachel L WinerLaura A Koutsky
Feb 13, 2013·International Journal of Cancer. Journal International Du Cancer·Mitzie-Ann DavisAaron A R Tobian
Jul 31, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Arundhati RaoCatherine Behrens
Nov 12, 2016·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Utaiwan UtaipatSuwat Chariyalertsak
Nov 8, 2013·Journal of Clinical Microbiology·Vikrant V SahasrabuddheNicolas Wentzensen
Dec 30, 2017·Experimental and Therapeutic Medicine·Ke ZhangJintao Li
Sep 7, 2019·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Rachael AdcockUNKNOWN New Mexico HPV Pap Registry Steering Committee
Nov 24, 2020·JNCI Cancer Spectrum·Gypsyamber D'SouzaCarole Fakhry

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