Human Papillomavirus Types 16 and 18 Early-expressed Proteins Differentially Modulate the Cellular Redox State and DNA Damage

International Journal of Biological Sciences
Alfredo Cruz-GregorioMarcela Lizano

Abstract

Oxidative stress has been proposed as a risk factor for cervical cancer development. However, few studies have evaluated the redox state associated with human papillomavirus (HPV) infection. The aim of this work was to determine the role of the early expressed viral proteins E1, E2, E6 and E7 from HPV types 16 and 18 in the modulation of the redox state in an integral form. Therefore, generation of reactive oxygen species (ROS), concentration of reduced glutathione (GSH), levels and activity of the antioxidant enzymes catalase and superoxide dismutase (SOD) and deoxyribonucleic acid (DNA) damage, were analysed in epithelial cells ectopically expressing the viral proteins. Our research shows that E6 oncoproteins decreased GSH and catalase protein levels, as well as its enzymatic activity, which was associated with an increase in ROS production and DNA damage. In contrast, E7 oncoproteins increased GSH, as well as catalase protein levels and its activity, which correlated with a decrease in ROS without affecting DNA integrity. The co-expression of both E6 and E7 oncoproteins neutralized the effects that were independently observed for each of the viral proteins. Additionally, the combined expression of E1 and E2 proteins increase...Continue Reading

Citations

Jun 23, 2018·International Journal of Molecular Sciences·Imelda Martínez-RamírezMarcela Lizano
Nov 15, 2018·Journal of Cellular Biochemistry·Safieh EbrahimiSeyed Isaac Hashemy
Apr 2, 2020·Frontiers in Pharmacology·Debora GrassoPierre Sonveaux
Apr 13, 2019·Scientific Reports·Philippe Paget-BaillyAurélie Baguet
Nov 19, 2019·Oxidative Medicine and Cellular Longevity·Saul Rassy CarneiroMaria da Conceição Nascimento Pinheiro
Oct 29, 2020·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Daciele Paola PreciAndréia Machado Cardoso
Feb 17, 2021·Reviews in Medical Virology·Ana Karina Aranda-RiveraJosé Pedraza-Chaverri
Aug 17, 2020·Life Sciences·Vaishali ChandelDhruv Kumar
Mar 13, 2021·Reviews in Medical Virology·Alfredo Cruz-Gregorio, Ana Karina Aranda-Rivera
May 6, 2021·Reviews in Medical Virology·Willem J SanderHester G O'Neill
Sep 11, 2019·Pathogens and Disease·Nima Hemmat, Hossein Bannazadeh Baghi
May 29, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Fernanda Costa Brandão BertiDanielle Malheiros
Jun 16, 2021·Free Radical Biology & Medicine·Alfredo Cruz-GregorioFrancisco Mendoza-Hoffmann
Oct 26, 2021·Reviews in Medical Virology·Alfredo Cruz-GregorioJosé Pedraza-Chaverri
Nov 24, 2021·Archives of Gynecology and Obstetrics·Burak SezginHakan Camuzcuoğlu

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