Human pathogenic Mycoplasma species induced cytokine gene expression in Epstein-Barr virus (EBV)-positive lymphoblastoid cell lines

Microbial Pathogenesis
E SchäffnerE Jacobs

Abstract

We addressed the question whether the in vitro interaction of two Epstein-Barr virus (EBV)-genome-positive B cell lines (EB-3 and HilB-gamma) with either Mycoplasma pneumoniae or M. hominis, with the <AIDS-related> mycoplasma species (M. fermentans, M. fermentans subsp. incognitus, M. penetrans, M. genitalium) or with mycoplasma species known to be mere commensals of the respiratory tract (M. orale and M. salivarium) would result in expression of mRNAs for IL-2, IL-2R, IL-4 and IL-6 as determined by reverse transcriptase (RT)-PCR after 4 and 24 h of cocultivation. The pattern of cytokine gene expression observed depended on (i) the origin of the transformed cell line, (ii) the pathogenicity of the Mycoplasma species, and (iii) the length of cocultivation. The EBV-immortalized lymphoblastoid cell line HilB-gamma showed mRNA expression for IL-2, IL-2-receptor, IL-4 and IL-6 peaking 24 h after stimulation with M. pneumoniae and all AIDS-related mycoplasma species tested. The Burkitt lymphoma cell line EB-3 showed a distinct and isolated strong II-2/IL-2 R-mRNA expression within 4 h after contact with the pathogenic and all of the AIDS related mycoplasma species. In neither EBV-containing cell line cytokine was gene expression dete...Continue Reading

References

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Citations

Mar 7, 2003·Microbial Pathogenesis·Jun YangDeborah F Talkington
Apr 28, 2004·Cytokine & Growth Factor Reviews·Jun YangDeborah F Talkington
Mar 20, 2004·Pharmacology & Therapeutics·Philip M HansbroPeter G Gibson
Jul 12, 2002·Pediatric Pulmonology·Susanna EspositoNicola Principi
Jan 7, 2017·Journal of Tropical Pediatrics·Biljana MedjoSlobodanka Djukic

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