Human Pax-5 C-terminal isoforms possess distinct transactivation properties and are differentially modulated in normal and malignant B cells.

The Journal of Biological Chemistry
Gilles A RobichaudRodney J Ouellette

Abstract

The transcription factor Pax-5 occupies a central role in B cell differentiation and has been implicated in the development of B cell lymphoma. The transcriptional activation function of Pax-5 requires an intact N-terminal DNA-binding domain and is strongly influenced by the C-terminal transactivation domain. We report the identification and characterization of five human Pax-5 isoforms, which occur through the alternative splicing of exons that encode for the C-terminal transactivation domain. These isoforms arise from the inclusion or exclusion of exon 7, exon 8, and/or exon 9. Three of the Pax-5 isoforms generate novel protein sequences rich in proline, serine, and threonine amino acids that are the hallmarks of transactivation domains. The Pax-5 isoforms are expressed in peripheral blood mononuclear cells, cancerous and non-cancerous B cell lines, as well as in primary B cell lymphoma tissue. Electrophoretic mobility shift assays demonstrate that the isoforms possess specific DNA binding activity and recognize the PAX-5 consensus binding sites. In reporter assays using the CD19 promoter, the transactivation properties of the various isoforms were significantly influenced by the changes in the C-terminal protein sequence. Fi...Continue Reading

References

Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·Z KozmikA Aguzzi
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·G ChalepakisP Gruss
Jun 1, 1994·Current Opinion in Genetics & Development·T Strachan, A P Read
Jan 1, 1994·Pharmacology & Therapeutics·P Tremblay, P Gruss
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·M BusslingerZ Kozmik
Aug 30, 1996·The Journal of Biological Chemistry·M S Lechner, G R Dressler
Dec 1, 1996·Current Opinion in Cell Biology·A MansouriP Gruss
Apr 11, 1997·The Journal of Biological Chemistry·P ZwolloR Pollock
Sep 23, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·E DahlR Balling
Nov 21, 1997·Biochemical and Biophysical Research Communications·C JaworskiG Wistow
May 7, 1998·The International Journal of Biochemistry & Cell Biology·F G Barr
Mar 22, 2000·Journal of Biotechnology·K RuscherA Meisel
Apr 5, 2000·The Journal of Biological Chemistry·C R KovacB K Birshtein
Aug 3, 2001·The EMBO Journal·S Weg-RemersH König
Sep 6, 2001·The Journal of Biological Chemistry·M LowenP Zwollo
Dec 26, 2001·Trends in Genetics : TIG·Neil Chi, Jonathan A Epstein
Mar 1, 2002·Current Opinion in Immunology·Michael SchebestaMeinrad Busslinger
Nov 1, 2002·Nature·Jack W Szostak
Nov 28, 2002·Blood·Nancy D BorsonPeter J Wettstein
Apr 5, 2003·Current Biology : CB·Eric C Lai
May 23, 2003·Human Molecular Genetics·Ramil N NurtdinovMikhail S Gelfand
Jun 17, 2003·Journal of Neurochemistry·Véronique SgambatoSteven E Hyman
Oct 11, 2003·Current Opinion in Genetics & Development·Kristen Johnson, Kathryn Calame
Dec 31, 2003·Trends in Genetics : TIG·Alexey G VitreschakMikhail S Gelfand
Apr 15, 2004·The Journal of Biological Chemistry·Matthew P Hardy, Luke A J O'Neill

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Citations

May 14, 2008·Journal of Molecular Evolution·Stephen Short, Linda Z Holland
Mar 7, 2006·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Nancy D BorsonPeter J Wettstein
Jun 26, 2013·Developmental and Comparative Immunology·Elizabeth MacMurrayPatty Zwollo
Oct 31, 2006·Omics : a Journal of Integrative Biology·Miroslava Cuperlovic-CulfRodney J Ouellette
Jul 18, 2009·Comparative and Functional Genomics·David TalaveraXavier de la Cruz
Jan 22, 2011·Developmental and Comparative Immunology·Patty Zwollo
Oct 20, 2006·American Journal of Clinical Pathology·Emina TorlakovicVivi Ann Flørenes
Jul 17, 2009·British Journal of Haematology·Alessandra SantoroMaurizio Arico
Sep 4, 2009·British Journal of Haematology·Jean-René ArseneauRodney J Ouellette
Jul 17, 2008·Journal of Cellular and Molecular Medicine·Qiuyu WangPatricia Kumar
Apr 13, 2011·British Journal of Haematology·Karin NebralSabine Strehl
Oct 24, 2006·Drug Discovery Today·Miroslava Cuperlovic-CulfRodney J Ouellette
Oct 18, 2016·The Indian Journal of Medical Research·Brij BhartiRajnikant Mishra
Nov 15, 2017·Cellular and Molecular Neurobiology·Shashank Kumar Maurya, Rajnikant Mishra
Jan 4, 2019·Carcinogenesis·Jason HarquailGilles A Robichaud
Jan 12, 2017·Oncotarget·Sami BenzinaGilles A Robichaud
Oct 17, 2019·Genes·Dhananjay HuilgolSonali Bhattacharjee
Jul 23, 2018·Journal of Mammary Gland Biology and Neoplasia·Jason HarquailGilles A Robichaud
Dec 1, 2011·Cancer Research·Pierre O'BrienGilles A Robichaud
Aug 23, 2020·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Wei LiZhaoling Cheng

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