Human Pegivirus in Patients with Encephalitis of Unclear Etiology, Poland

Emerging Infectious Diseases
Iwona Bukowska-OśkoT Laskus

Abstract

Human pegivirus (HPgV), previously called hepatitis G virus or GB virus C, is a lymphotropic virus with undefined pathology. Because many viruses from the family Flaviviridae, to which HPgV belongs, are neurotropic, we studied whether HPgV could infect the central nervous system. We tested serum and cerebrospinal fluid samples from 96 patients with a diagnosis of encephalitis for a variety of pathogens by molecular methods and serology; we also tested for autoantibodies against neuronal antigens. We found HPgV in serum and cerebrospinal fluid from 3 patients who had encephalitis of unclear origin; that is, all the markers that had been tested were negative. Single-strand confirmation polymorphism and next-generation sequencing analysis revealed differences between the serum and cerebrospinal fluid-derived viral sequences, which is compatible with the presence of a separate HPgV compartment in the central nervous system. It is unclear whether HPgV was directly responsible for encephalitis in these patients.

References

May 1, 1992·Journal of Neuropathology and Experimental Neurology·W F HickeyH Lassmann
Jan 1, 1991·Advances in Virus Research·V I Agol
Feb 1, 1991·Journal of Neuroscience Research·W F HickeyH Kimura
Jun 1, 1995·Nature Medicine·J N SimonsI K Mushahwar
Mar 21, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M G PessoaT L Wright
Aug 26, 1998·Liver·M J MphahleleW F Carman
Jan 11, 2000·Clinical Microbiology Reviews·P LeyssenJ Neyts
Feb 17, 2000·The Journal of General Virology·D B SmithP Simmonds
Sep 8, 2001·The New England Journal of Medicine·J XiangJ T Stapleton
Mar 12, 2003·Journal of Medical Virology·Gunn Eli Kimo JørgensenJohn Inge Johnsen
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Dec 21, 2004·Journal of Neurology, Neurosurgery, and Psychiatry·N W S DaviesP Muir
Aug 7, 2007·Neuropsychological Rehabilitation·Julia Granerod, Natasha S Crowcroft
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Sep 18, 2009·Journal of Viral Hepatitis·Emma L Mohr, Jack T Stapleton
Nov 12, 2009·International Journal of Cancer. Journal International Du Cancer·Mel KrajdenJohn J Spinelli
Oct 26, 2010·Travel Medicine and Infectious Disease·Daniel RůžekOliver Donoso Mantke
Feb 14, 2012·Trends in Microbiology·Nirjal Bhattarai, Jack T Stapleton
Jun 13, 2012·Microbiology and Molecular Biology Reviews : MMBR·Esteban DomingoCelia Perales
Jun 15, 2012·Wiener medizinische Wochenschrift·Reinhard Kaiser
Apr 29, 2015·Current HIV/AIDS Reports·Maria M BednarRonald Swanstrom
Feb 13, 2016·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Helena FridholmAnders Fomsgaard
May 5, 2017·Nucleic Acids Research·Martin Closter JespersenPaolo Marcatili
Oct 14, 2017·Molecular Biology and Evolution·Julio RozasAlejandro Sánchez-Gracia

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Citations

Apr 6, 2019·Current Opinion in Infectious Diseases·Catherine F HoulihanJudith Breuer
May 6, 2020·PloS One·Ana Beatriz Figueiredo de LimaLuiz Fernando Almeida Machado
Aug 21, 2021·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Marianne GraningerElisabeth Puchhammer-Stöckl

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Methods Mentioned

BETA
PCR
reverse transcription PCR
Illumina sequencing

Software Mentioned

FASTX
R scripts
DnaSP
A Artifacts Filter
ClustalX
FASTQ
Mfold

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