Human Periodontal Ligament- and Gingiva-derived Mesenchymal Stem Cells Promote Nerve Regeneration When Encapsulated in Alginate/Hyaluronic Acid 3D Scaffold

Advanced Healthcare Materials
Sahar AnsariAlireza Moshaverinia

Abstract

Repair or regeneration of damaged nerves is still a challenging clinical task in reconstructive surgeries and regenerative medicine. Here, it is demonstrated that periodontal ligament stem cells (PDLSCs) and gingival mesenchymal stem cells (GMSCs) isolated from adult human periodontal and gingival tissues assume neuronal phenotype in vitro and in vivo via a subcutaneous transplantation model in nude mice. PDLSCs and GMSCs are encapsulated in a 3D scaffold based on alginate and hyaluronic acid hydrogels capable of sustained release of human nerve growth factor (NGF). The elasticity of the hydrogels affects the proliferation and differentiation of encapsulated MSCs within scaffolds. Moreover, it is observed that PDLSCs and GMSCs are stained positive for βIII-tubulin, while exhibiting high levels of gene expression related to neurogenic differentiation (βIII-tubulin and glial fibrillary acidic protein) via quantitative polymerase chain reaction (qPCR). Western blot analysis shows the importance of elasticity of the matrix and the presence of NGF in the neurogenic differentiation of encapsulated MSCs. In vivo, immunofluorescence staining for neurogenic specific protein markers confirms islands of dense positively stained structures...Continue Reading

References

Jan 14, 1975·Biochemistry·R U MargolisC Preti
Feb 1, 1997·Molecular Neurobiology·S Y Fu, T Gordon
Aug 10, 2000·Journal of Neuroscience Research·D WoodburyI B Black
Oct 4, 2000·Physiological Reviews·C E Bandtlow, D R Zimmermann
Jan 3, 2001·Seminars in Surgical Oncology·G R Evans
Jul 31, 2002·Journal of Dental Research·S GronthosS Shi
Sep 3, 2002·Journal of Neuroscience Research·Marina Romero-RamosMarie-Françoise Chesselet
Nov 16, 2002·Journal of Microencapsulation·B L StrandG Skjåk-Baek
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Masako MiuraSongtao Shi
Aug 19, 2003·Biomaterials·Jeanie L Drury, David J Mooney
Sep 6, 2003·Birth Defects Research. Part C, Embryo Today : Reviews·Cheryl B Knudson
May 26, 2004·Neuroscience Letters·Mel TohillGiorgio Terenghi
Dec 22, 2006·PloS One·Wataru SonoyamaSongtao Shi
Nov 20, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Qunzhou ZhangAnh D Le
May 11, 2010·Journal of Periodontology·Tomas I MitranoAntonio Sanz
Sep 8, 2010·Current Topics in Developmental Biology·Jianing LiuAmy Wagers
May 31, 2011·Frontiers in Bioscience (Scholar Edition)·Marnie Preston, Larry S Sherman
Nov 30, 2011·Progress in Polymer Science·Kuen Yong Lee, David J Mooney
Dec 1, 2012·Stem Cells Translational Medicine·David Antonio CantuWeiyuan John Kao
Jul 23, 2013·Journal of Dental Research·X XuS Shi
Oct 3, 2013·International Review of Neurobiology·Júlia Teixeira OliveiraAna Maria Blanco Martinez
Nov 26, 2013·Tissue Engineering. Part C, Methods·Ziying YinRichard L Magin
Jan 15, 2014·Tissue Engineering. Part C, Methods·Isobel MassieG John Morris

❮ Previous
Next ❯

Citations

Feb 27, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mariana I NevesCristina C Barrias
Nov 9, 2019·Current Stem Cell Research & Therapy·Xudong Gao, Zhengguo Cao
May 21, 2020·Scientific Reports·Samuel Rodríguez ZorrillaMario Pérez-Sayáns
Oct 23, 2019·Stem Cells International·Tao ZhouXin Zhou
Jul 2, 2019·International Journal of Molecular Medicine·Xiaoyan YuanJie Long
Apr 28, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jiandong HanXiangru Feng
Oct 10, 2020·World Journal of Stem Cells·Cornelia Larissa Granz, Ali Gorji
Nov 17, 2020·Advanced Healthcare Materials·Sanja AveicHorst Fischer
Jan 23, 2021·Journal of Personalized Medicine·Yu Gyung KimDo-Yeon Kim
May 29, 2021·Stem Cells International·Jingqiu ChenJun Zhang
Jun 18, 2021·World Journal of Stem Cells·Jing-Yao YinDan-Dan Ma
Jul 2, 2021·International Journal of Oral Science·Sevda Pouraghaei SevariAlireza Moshaverinia
Jan 19, 2021·ACS Biomaterials Science & Engineering·Sevda PouraghaeiAlireza Moshaverinia

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.