Human Periodontal Ligament-Derived Stem Cells Promote Retinal Ganglion Cell Survival and Axon Regeneration After Optic Nerve Injury

Stem Cells
Ling-Ping CenChi Pui Pang

Abstract

Optic neuropathies are the leading cause of irreversible blindness and visual impairment in the developed countries, affecting more than 80 million people worldwide. While most optic neuropathies have no effective treatment, there is intensive research on retinal ganglion cell (RGC) protection and axon regeneration. We previously demonstrated potential of human periodontal ligament-derived stem cells (PDLSCs) for retinal cell replacement. Here, we report the neuroprotective effect of human PDLSCs to ameliorate RGC degeneration and promote axonal regeneration after optic nerve crush (ONC) injury. Human PDLSCs were intravitreally injected into the vitreous chamber of adult Fischer rats after ONC in vivo as well as cocultured with retinal explants in vitro. Human PDLSCs survived in the vitreous chamber and were maintained on the RGC layer even at 3 weeks after ONC. Immunofluorescence analysis of βIII-tubulin and Gap43 showed that the numbers of surviving RGCs and regenerating axons were significantly increased in the rats with human PDLSC transplantation. In vitro coculture experiments confirmed that PDLSCs enhanced RGC survival and neurite regeneration in retinal explants without inducing inflammatory responses. Direct cell-cell ...Continue Reading

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Citations

Aug 23, 2018·Stem Cells·Majlinda Lako
Jul 13, 2019·Developmental Neurobiology·George G BastakisMaria Savvaki
Jun 20, 2019·Stem Cells and Development·Atsushi TomokiyoHidefumi Maeda
Dec 7, 2019·Current Opinion in Neurology·Kimberly K GokoffskiPhillip Lam
Jul 3, 2020·Chemical Communications : Chem Comm·Man-Man XuShuang-Quan Zang
Aug 6, 2019·Oxidative Medicine and Cellular Longevity·Yaping YangTsz Kin Ng
Oct 2, 2019·Neural Regeneration Research·Alessandra PisciottaGianluca Carnevale
Aug 15, 2018·Neural Regeneration Research·Ling-Ping Cen, Tsz Kin Ng
May 3, 2019·Investigative Ophthalmology & Visual Science·Virpi AlarautalahtiSoile Nymark
Mar 19, 2020·Stem Cells International·Li-Li ZhouK M Fawzy El-Sayed
Oct 27, 2020·Investigative Ophthalmology & Visual Science·Yuxun ShiJingjing Huang
Dec 4, 2020·Neural Regeneration Research·Jia-Jian LiangLing-Ping Cen
Oct 28, 2020·International Journal of Molecular Sciences·Ayaka EdoMasayo Takahashi
Mar 23, 2021·Frontiers in Bioengineering and Biotechnology·Yifan XiangHaotian Lin
Apr 1, 2021·Experimental Neurology·Yu-Fen LiuLing-Ping Cen
Jul 11, 2021·Proceedings of the National Academy of Sciences of the United States of America·Ling-Ping CenChi Pui Pang

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