Human peripheral blood endothelial progenitor cells synthesize and express functionally active tissue factor

Thrombosis Research
R Di StefanoAlessandro Celi

Abstract

Endothelial progenitor cells are circulating cells able to home to sites of vascular damage and to contribute to the revascularization of ischemic areas. We evaluated whether endothelial progenitor cells synthesize tissue factor, a procoagulant protein also involved in angiogenesis. Endothelial progenitor cells were obtained from the peripheral blood mononuclear fraction of normal donors and cultured in endothelial medium supplemented with specific growth factors. The procoagulant activity expressed by cells disrupted by freeze-thaw cycles was assessed by a one stage clotting assay. Tissue factor mRNA expression was evaluated by RT-PCR. Endothelial progenitor cells do not express procoagulant activity in baseline conditions. However, lipopolysaccharide induces the expression of procoagulant activity. The effect is dose-dependent and reaches statistical significance at 100 ng/mL lipopolysaccharide. Inhibition with an anti-tissue factor antibody and amplification of cDNA with primers based on the tissue factor sequence confirm the identity of this activity with tissue factor. The kinetics of tissue factor expression by endothelial progenitor cells is identical to that of human umbilical vein endothelial cells showing maximal acti...Continue Reading

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Jun 8, 2010·Stem Cell Reviews and Reports·Chahrazad MoubarikPascale Pisano
Apr 28, 2011·Naunyn-Schmiedeberg's Archives of Pharmacology·Massimo Dal MontePaola Bagnoli
Apr 14, 2012·Thrombosis Research·Eleonora Da PozzoRossella Di Stefano
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Jan 22, 2019·Molecular Medicine Reports·Lili PanNing Xu
Aug 6, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Jialin PanCong Lin

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