Human peripheral blood lymphocyte activation by protein A from Staphylococcus aureus.

Infection and Immunity
W Y ChenH H Fudenberg

Abstract

Mitogenesis and polyclonal immunoglobulin production in peripheral blood lymphocyte cultures activated with Formalin-fixed or autoclaved protein A-containing Staphylococcus aureus were studied. Direct evidence for a dissociation between cell proliferation and polyclonal immunoglobulin production was found, in that S. aureus was not mitogenic after being autoclaved but retained the ability to stimulate B cells to produce immunoglobulin. Trypsin-treated S. aureus lost its binding site for immunoglobulin G, but its mitogenicity was not altered; thus, the protein A binding site for immunoglobulin G on the bacterial cell wall is not required for the stimulation of lymphocyte proliferation. Our data also show a dissociation between cell proliferation and polyclonal immunoglobulin production induced by protein A coupled to Sepharose CL-4B. These results suggest the presence of three distinct active sites on the protein A molecule: one that binds immunoglobulin G molecules, one that stimulates cell proliferation, and one that stimulates polyclonal immunoglobulin production.

References

Jan 1, 1978·European Journal of Immunology·O Ringdén, Rynnel-Dagöö
Mar 1, 1976·European Journal of Immunology·A ForsgrenH Wigzell
Jul 1, 1973·Clinical Immunology and Immunopathology·Z BentwichH G Kunkel

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Citations

Jan 1, 1991·Cancer Immunology, Immunotherapy : CII·R A LindemannR K Gupta
Jan 1, 1985·Critical Reviews in Microbiology·F Quimby, H T Nguyen
Jun 1, 1986·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology·P AasjordR Matre
Sep 14, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yue TongHong Tian

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