Human Placenta-Derived Mesenchymal Stem Cells Reduce Mortality and Hematoma Size in a Rat Intracerebral Hemorrhage Model in an Acute Phase

Stem Cells International
Bo Young ChoiTae Nyoung Chung

Abstract

Intracerebral hemorrhage (ICH) is a critical disease, highly associated with mortality and morbidity. Several studies have demonstrated the beneficial effect of mesenchymal stem cells (MSCs) on ICH, mostly focused on their mid-to-long-term effect. Acute hematoma expansion is one of the most important prognostic factors of ICH. We hypothesized that MSCs would decrease mortality and hematoma size in acute ICH, based on the findings of a few recent researches reporting their effect on blood-brain barrier and endothelial integrity. Rat ICH models were made using bacterial collagenase. One hour after ICH induction, the rats were randomly divided into MSC-treated and control groups. Mortality, hematoma volume, ventricular enlargement, brain edema, and degenerating neuron count were compared at 24 hours after ICH induction. Expression of tight junction proteins (ZO-1, occludin) and coagulation factor VII mRNA was also compared. Mortality rate (50% versus 8.3%), hematoma size, ventricular size, hemispheric enlargement, and degenerating neuron count were significantly lower in the MSC-treated group (p = 0.034, 0.038, 0.001, 0.022, and <0.001, resp.), while the expression of ZO-1 and occludin was higher (p = 0.007 and 0.012). Administrat...Continue Reading

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Citations

May 18, 2020·Molecular Biology Reports·Abel Po-Hao HuangDar-Ming Lai
Apr 13, 2021·Frontiers in Cellular Neuroscience·Yu-Hua GongBo-Chu Wang
May 1, 2021·Reproductive Biology·Prasad Pethe, Vaijayanti Kale

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Methods Mentioned

BETA
PCR
Protein Assay
Fluorescence

Software Mentioned

ImageJ
SPSS statistics
Power

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