Human placental trophoblast as an in vitro model for tumor progression

Canadian Journal of Physiology and Pharmacology
P K LalaC Chakraborty

Abstract

The human placenta is a highly invasive tumor-like structure in which a subpopulation of placental trophoblast cells known as the "extravillous trophoblast" (EVT) invades the uterine decidua and its vasculature to establish adequate fetal-maternal exchange of molecules. By utilizing in vitro-propagated short-lived EVT cell lines we found that molecular mechanisms responsible for their invasiveness are identical to those of cancer cells; however, unlike cancer cells, their proliferation, migration, and invasiveness in situ are stringently controlled by decidua-derived transforming growth factor (TGF)-beta. By SV40T antigen transfection of normal EVT cells followed by a forced crisis regimen in culture we produced an immortalized premalignant derivative that is hyperproliferative, hyperinvasive, and deficient in gap-junctional intercellular communication. Both premalignant and malignant EVT (JAR and JEG-3 choriocarcinoma) cell lines were found to be TGF-beta-resistant. Using these cell lines, we investigated genetic changes responsible for transition of the normal EVT cells to premalignant and malignant phenotype. Hyperinvasiveness in both cases resulted from a downregulation of tissue inhibitor of metalloprotease (TIMP)-1 and pl...Continue Reading

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Citations

Nov 25, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Amy ChuiPadma Murthi
Mar 20, 2012·Cell and Tissue Research·Toshihiko EzashiR Michael Roberts
Sep 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·André A Fernandez, Molly R Morris
Jun 12, 2014·Journal of Reproductive Immunology·Perrie F O'Tierney-Ginn, Gendie E Lash
Sep 6, 2011·Molecular and Cellular Endocrinology·Dave LanoixCathy Vaillancourt
Jun 9, 2007·Molecular and Cellular Endocrinology·Junko TanakaHideko Sone
Jun 10, 2006·Biological Reviews of the Cambridge Philosophical Society·Bernard J Crespi, Kyle Summers
Jan 18, 2005·Experimental Eye Research·Zhiyu WangMichelle Kurpakus-Wheater
May 17, 2006·Trends in Ecology & Evolution·Bernard Crespi, Kyle Summers
Oct 3, 2015·American Journal of Obstetrics and Gynecology·Vania JanuarRichard Saffery
Feb 27, 2015·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·T P H NguyenP Murthi
Nov 6, 2014·Nutrition and Cancer·Colin A Ross
Feb 6, 2009·Placenta·M K ChrostowskiJ F Padbury
Aug 2, 2017·International Journal of Molecular Sciences·Yao YeViktoria von Schönfeldt
Apr 20, 2004·Molecular Reproduction and Development·Dong QianCheng Zhu
Jul 25, 2017·Journal of Molecular Endocrinology·Fabien DuvalMarie-Noëlle Dieudonné
Nov 22, 2018·Molecular Human Reproduction·Sonia C DaSilva-ArnoldNicholas P Illsley
Jun 8, 2012·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Quan NaWeiwei Song
Dec 15, 2011·Endocrinology·York Hunt NgPeter C K Leung
May 11, 2018·Nutrition and Cancer·Colin A Ross
Sep 12, 2020·Placenta·Fengge WangDongmei Man
May 8, 2021·Placenta·Peeyush K LalaChidambra Halari
Sep 1, 2021·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·Kun DongZhiwei Zhang

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