PMID: 3766720Oct 1, 1986Paper

Human platelet osmotic water and nonelectrolyte transport

The American Journal of Physiology
M M Meyer, A S Verkman

Abstract

The osmotic water (Pf) and nonelectrolyte permeability (Ps) properties of human platelets were characterized using the stopped-flow light-scattering technique. At 37 degrees C, Pf = 0.007 +/- 0.001 cm/s, the urea reflection coefficient (sigma urea) = 0.95 +/- 0.04, and Ps for a series of permeant nonelectrolytes was (in cm X s-1 X 10(-6)) 2.1 (urea), 3.5 (glycerol), 3.8 (thiourea), 17 (ethylene glycol), 18 (acetamide), 23 (formamide), and 24 (butyramide). Pf did not depend on the size of the osmotic gradient or on the direction of volume flow. Mercurial sulfhydryl reagents did not inhibit osmotic water transport, and phloretin and phenylurea did not inhibit urea transport. There was a discontinuity in the temperature dependence for both Pf and urea permeability (P urea) at 36 degrees C; enthalpy (delta H) = 25 (greater than 36 degrees C) and 4.4 kcal/mol (less than 36 degrees C) for Pf, and delta H = 26 (greater than 36 degrees C) and 7 kcal/mol (less than 36 degrees C) for P urea. In contrast to the facilitated water and urea transport systems in the red blood cell, these results suggest that the mechanism for water and urea transport in the platelet is primarily by diffusion through membrane phospholipid. A computer-simulated...Continue Reading

References

Dec 31, 1979·The Journal of Membrane Biology·J M Diamond
Dec 5, 1985·Biochimica Et Biophysica Acta·J A DixA S Verkman
May 1, 1985·Journal of Cellular Physiology·W J ArmitageC J Hunt
Jan 1, 1973·Progress in Biophysics and Molecular Biology·R I Sha'afi, C M Gary-Bobo
Jun 1, 1973·The Journal of General Physiology·W R GaleyA K Solomon
Sep 1, 1971·The Journal of General Physiology·R I Sha'afiA K Solomon
Sep 1, 1968·The Journal of Physiology·P Fantl
Feb 1, 1983·The Journal of General Physiology·H J MlekodayD G Levitt
Feb 1, 1983·The Journal of General Physiology·D G Levitt, H J Mlekoday
Apr 1, 1980·Physiological Reviews·R Fettiplace, D A Haydon
May 1, 1982·The Journal of General Physiology·J Brahm
Jan 8, 1981·Biochimica Et Biophysica Acta·M Steiner
May 1, 1981·The Journal of General Physiology·T C Terwilliger, A K Solomon
Sep 1, 1960·The Journal of General Physiology·D A GOLDSTEIN, A K SOLOMON

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Citations

Sep 1, 1988·The Journal of Membrane Biology·A S Verkman, S K Masur
Jul 2, 2010·Plant Molecular Biology·Jorge BellatiGabriela Amodeo
Sep 1, 1990·The Journal of Membrane Biology·R B Zhang, A S Verkman
Jan 1, 1986·The Journal of Membrane Biology·N P Illsley, A S Verkman
May 11, 1992·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·H W Harris
May 9, 1998·The Journal of Biological Chemistry·N RoudierF Tacnet
May 1, 1987·Pflügers Archiv : European journal of physiology·P Y Chen, A S Verkman
Oct 1, 1993·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·H W HarrisM L Zeidel
Jan 1, 1987·The Journal of Membrane Biology·M M Meyer, A S Verkman
Sep 5, 2008·European Biophysics Journal : EBJ·Karina AllevaGabriela Amodeo
Oct 29, 2011·Journal of the American Society of Nephrology : JASN·Gautam Bhave, Eric G Neilson
Jan 21, 1992·Biochemistry·M L ZeidelA Carruthers

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