Human platelets express gangliosides with LKE activity and ABH blood group activity

Transfusion
L L CoolingT A Koerner

Abstract

Platelets express several neutral glycosphingolipids with ABH and P blood group activity that may play a role in infectious, autoimmune, and alloimmune thrombocytopenia. In RBCs, sialylated glycosphingolipids or gangliosides with blood group activity have also been reported. To determine whether similar antigens are expressed by platelets, the total platelet ganglioside fraction was isolated and screened for blood-group-active glycosphingolipids. Platelet gangliosides were isolated by organic extraction, base hydrolysis, anion exchange, silicic acid, and high-performance liquid chromatography. Gangliosides were identified and characterized by high-performance thin-layer chromatography-immunostaining with blood group-specific MoAbs and glycosidase digestion. Group A, but not group O, platelets express five gangliosides with group A activity. Of five A MoAbs and lectins examined, only MoAbs Birma-1 and MHO4 recognized all five sialyl A bands. The sialyl A bands were sensitive to endoglycoceramidase and neuraminidase. One sialyl A band may represent a branched ganglioside with sialyl-I and group A activity. Platelets also express an LKE-active ganglioside consistent with sialyl-galactosylgloboside. In addition to sialyl-iI and sia...Continue Reading

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Citations

Jul 14, 2001·Transfusion·L L Cooling, K Kelly
Jun 6, 2012·Stem Cells International·Sally A Boxall, Elena Jones
Jun 6, 2015·Arteriosclerosis, Thrombosis, and Vascular Biology·Ming ZhongMuredach P Reilly
Dec 23, 2004·Blood·Laura L W CoolingJohn D Olson
Aug 5, 2020·International Journal of Molecular Sciences·Keisuke KomatsuyaKohji Kasahara

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