Human progenitor-derived endothelial cells vs. venous endothelial cells for vascular tissue engineering: an in vitro study

Journal of Tissue Engineering and Regenerative Medicine
Noélie B ThebaudLaurence Bordenave

Abstract

The isolation of endothelial progenitor cells from human peripheral blood generates a great hope in vascular tissue engineering because of particular benefit when compared with mature endothelial cells. We explored the capability of progenitor-derived endothelial cells (PDECs) to line fibrin and collagen scaffolds in comparison with human saphenous and umbilical cord vein endothelial cells (HSVECs and HUVECs): (a) in a static situation, allowing definition of the optimal cell culture conditions with different media and cell-seeding densities to check cell behaviour; (b) under shear stress conditions (flow chambers or tubular vascular constructs), allowing investigation of cell response and mRNA expression on both substrates by oligonucleotide microarray analysis and quantitative real-time PCR. Well characterized PDECs: (a) could not be expanded adequately with the usual mature ECs culture media; (b) were able to colonize and grow on fibrin glue; (c) exhibited higher resistance to oxidative stress than HSVECs and HUVECs; (d) withstood physiological shear stress when lining both substrates in flow chambers, and their gene expression was regulated; (e) colonized a collagen-impregnated vascular prosthesis and were able to sense mec...Continue Reading

References

Nov 1, 1993·Journal of Biomedical Materials Research·L BordenaveJ M Anderson
Sep 4, 1999·Endothelium : Journal of Endothelial Cell Research·D KaiserP Friedl
Jul 8, 2000·The Journal of Thoracic and Cardiovascular Surgery·H R LaubeW Konertz
Nov 30, 2000·Biochemical and Biophysical Research Communications·B BartlingH Morawietz
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·G Garcia-CardeñaM A Gimbrone
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·A GiuliettiC Mathieu
May 7, 2003·Progress in Biophysics and Molecular Biology·Nitzan ResnickEfrat Wofovitz
Oct 29, 2003·Circulation·Kathy K Griendling, Garret A FitzGerald
Dec 31, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Jin HurYoung-Bae Park
Aug 21, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Tongrong HeZvonimir S Katusic
Apr 13, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Carmen UrbichStefanie Dimmeler
Dec 21, 2005·Pathologie-biologie·Dina S VaraAlexander M Seifalian
Feb 28, 2006·Tissue Engineering·Philippe FernandezLaurence Bordenave
May 13, 2006·Journal of Zhejiang University. Science. B·Si-feng TaoHong Yu
Jan 16, 2007·Journal of Thrombosis and Thrombolysis·Zhen YangJun Tao
Feb 20, 2007·The Annals of Thoracic Surgery·Davide GabbieriWolfgang Konertz
Feb 24, 2007·Biomaterials·Alison P McGuigan, Michael V Sefton
May 5, 2007·Macromolecular Bioscience·Frédéric CouetDiego Mantovani
Nov 27, 2007·Experimental Hematology·Cátia IgrejaSérgio Dias
Mar 25, 2008·Trends in Biotechnology·Jerome G RoncalliDouglas W Losordo
May 3, 2008·Expert Review of Medical Devices·Laurence BordenaveCharles Baquey
May 31, 2008·Expert Review of Cardiovascular Therapy·Glenn Marsboom, Stefan Janssens
Jun 17, 2008·Cells, Tissues, Organs·Faisal M ShaikhTim M McGloughlin
Oct 1, 2008·International Journal of Cardiology·Giulio PompilioPaolo Biglioli
Oct 25, 2008·Regenerative Medicine·Cheen P KhooMalcolm R Alison
Dec 27, 2008·Journal of Vascular Surgery·Manfred DeutschMartin Grabenwoeger
Feb 12, 2009·Journal of Thrombosis and Haemostasis : JTH·J D Pearson
Jun 17, 2009·Journal de la Société de biologie·David M Smadja, Pascale Gaussem

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Citations

May 24, 2011·Tissue Engineering. Part a·Katherine A AhmannRobert T Tranquillo
Dec 3, 2013·Journal of Biomedical Materials Research. Part a·Lila RamiLaurence Bordenave
Jul 16, 2013·Journal of Biomedical Materials Research. Part a·Christian HessStephan Barcikowski
Oct 6, 2012·Journal of Tissue Engineering and Regenerative Medicine·Wolfgang HolnthonerHeinz Redl
Sep 24, 2013·Biomaterials·Murielle RémyLaurence Bordenave
Aug 4, 2019·Journal of Biomedical Materials Research. Part a·Vera GuduricSylvain Catros
Jun 4, 2020·International Journal of Molecular Sciences·Maria Cristina VinciGiulio Pompilio

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