PMID: 6982098Nov 1, 1982Paper

Human prostate androgen receptor quantitation: effects of temperature on assay parameters

Cancer Research
S A ShainD L Lamm

Abstract

When cytoplasmic extracts of human prostatic tissues were split to permit quantitation of total androgen receptor (RCT) content by saturation analysis at 15 degrees and 2 degrees, we observed that 30% (10 of 32) of the specimens yielded statistically increased values for RCT following incubation at 15 degrees as compared to 2 degrees. Considering only those specimens (13 of 32) showing statistically differentiated RCT yield, 77% (10 of 13) yielded greater RCT content following incubation at 15 degrees. The families of association constants (Ka) obtained for RCT determinations at 2 degrees and 15 degrees were not statistically differentiated. The increased yield of RCT content determined at 15 degrees was 95% (mean) and 20 to 350% (range). Nuclear androgen receptor content determined at 15 degrees was greater for 25% (2 of 8) of the patient specimens when compared to split determinations performed at 2 degrees. Incubation of nuclear extracts at 15 degrees resulted in a significant 3-fold reduction in receptor Ka for methyltrienolone (R1881). This did not appear to affect assay precision. These studies showed that incubation at 15 degrees is preferable to incubation at 2 degrees for quantitation of RCT and nuclear androgen recept...Continue Reading

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