Human prostate sphere-forming cells represent a subset of basal epithelial cells capable of glandular regeneration in vivo.

The Prostate
I GarrawayOwen N Witte

Abstract

Prostate stem/progenitor cells function in glandular development and maintenance. They may be targets for tumor initiation, so characterization of these cells may have therapeutic implications. Cells from dissociated tissues that form spheres in vitro often represent stem/progenitor cells. A subset of human prostate cells that form prostaspheres were evaluated for self-renewal and tissue regeneration capability in the present study. Prostaspheres were generated from 59 prostatectomy specimens. Lineage marker expression and TMPRSS-ERG status was determined via immunohistochemistry and fluorescence in situ hybridization (FISH). Subpopulations of prostate epithelial cells were isolated by cell sorting and interrogated for sphere-forming activity. Tissue regeneration potential was assessed by combining sphere-forming cells with rat urogenital sinus mesenchyme (rUGSM) subcutaneously in immunocompromised mice. Prostate tissue specimens were heterogeneous, containing both benign and malignant (Gleason 3-5) glands. TMPRSS-ERG fusion was found in approximately 70% of cancers examined. Prostaspheres developed from single cells at a variable rate (0.5-4%) and could be serially passaged. A basal phenotype (CD44+CD49f+CK5+p63+CK8-AR-PSA-) w...Continue Reading

References

Nov 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·R SnoekN Bruchovsky
Aug 11, 1998·Differentiation; Research in Biological Diversity·S W HaywardG R Cunha
Sep 25, 1999·Journal of Cellular Physiology·D R BlatchfordC J Wilde
Dec 18, 2001·The Journal of Pathology·G J van LeendersJ A Schalken
Jun 28, 2002·Cancer Cell·Judah Folkman
May 21, 2003·Genes & Development·Gabriela DontuMax S Wicha
Dec 4, 2003·Brain Research·Alessandra BezEugenio Agostino Parati
Jul 1, 2004·Journal of Cell Science·Gavin D RichardsonAnne T Collins
Sep 16, 2004·The Prostate·Isla P GarrawayRobert E Reiter
Sep 21, 2004·Oncogene·Muhammad Al-Hajj, Michael F Clarke
Oct 29, 2005·Science·Scott A TomlinsArul M Chinnaiyan
Dec 3, 2005·Cancer Research·Anne T CollinsNorman J Maitland
Mar 8, 2006·Nature Medicine·Kornelia Polyak, William C Hahn
Apr 1, 2006·Science·Kateri A Moore, Ihor R Lemischka
Apr 25, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Anne T Collins, Norman J Maitland
Aug 22, 2006·Molecular Carcinogenesis·Dean G TangCollene Jeter
Sep 23, 2006·The New England Journal of Medicine·Craig T JordanMark Noble
Dec 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Devon A LawsonOwen N Witte
Nov 3, 2007·Developmental Biology·Xudong ShiWade Bushman
Dec 3, 2008·Cancer Research·Donald J Vander GriendJohn T Isaacs
Dec 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Andrew S GoldsteinOwen N Witte

❮ Previous
Next ❯

Citations

Sep 20, 2012·The Journal of Biological Chemistry·Michael W HanceJennifer S Isaacs
Jul 31, 2010·Science·Andrew S GoldsteinOwen N Witte
Jan 26, 2013·The Journal of Clinical Investigation·Toshiaki YoshiokaFilippo G Giancotti
May 30, 2013·PloS One·Hongjuan ZhaoDonna M Peehl
Feb 18, 2016·Stem Cells International·Alastair H Davies, Amina Zoubeidi
Aug 27, 2015·Endocrine-related Cancer·Douglas W Strand, Andrew S Goldstein
Sep 6, 2015·Endocrine-related Cancer·Maho Shibata, Michael M Shen
Apr 26, 2011·Biochimica Et Biophysica Acta·Vincenzo Graziano, Vincenzo De Laurenzi
Apr 11, 2015·Acta histochemica·Kei-ichiro UemuraKei-ichiro Nakamura
Apr 26, 2019·Cells·Omar Salem, Carsten G Hansen
Feb 23, 2020·The Prostate·Anna M GleaveYuzhuo Wang
Jul 6, 2014·International Journal of Oncology·Viviana CastilloEnrique A Castellon
Sep 23, 2014·Nature Cell Biology·Chee Wai ChuaMichael M Shen
Jun 3, 2015·The Journal of Biological Chemistry·Yanqing HuangFen Wang
Jan 23, 2016·Nature Protocols·Jarno DrostHans Clevers
Apr 16, 2016·Oncotarget·Guillaume BrocquevilleRoland P Bourette
Dec 31, 2016·Oncotarget·Krystal D NolanJennifer S Isaacs
Jul 24, 2020·The Journal of Clinical Investigation·Fei LiDong Gao
Apr 13, 2017·Frontiers in Pharmacology·Wei QinMeng Zhao
Dec 20, 2014·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Annie R A McDougallMegan J Wallace
Sep 25, 2015·Biology of Reproduction·Marion MandonDaniel G Cyr
Aug 6, 2020·Frontiers in Molecular Biosciences·Ritika TiwariBushra Ateeq
Nov 7, 2015·Current Protocols in Stem Cell Biology·Flora ClémentVéronique Maguer-Satta
Jul 12, 2017·Stem Cells International·Koran S Harris, Bethany A Kerr

❮ Previous
Next ❯

Methods Mentioned

BETA
xenografts
Infection
FCS
FACS

Software Mentioned

Diva

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

© 2022 Meta ULC. All rights reserved